Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance
AffiliationKeele University, United Kingdom; Drexel University College of Medicine, Philadelphia, USA
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AbstractAIM: To achieve high and sustained magnetic particle loading in a proliferative and endocytotically active neural transplant population (astrocytes) through tailored magnetite content in polymeric iron oxide particles. MATERIALS & METHODS: MPs of varying magnetite content were applied to primary-derived rat cortical astrocytes ± static/oscillating magnetic fields to assess labeling efficiency and safety. RESULTS: Higher magnetite content particles display high but safe accumulation in astrocytes, with longer-term label retention versus lower/no magnetite content particles. Magnetic fields enhanced loading extent. Dynamic live cell imaging of dividing labeled astrocytes demonstrated that particle distribution into daughter cells is predominantly 'asymmetric'. CONCLUSION: These findings could inform protocols to achieve efficient MP loading into neural transplant cells, with significant implications for post-transplantation tracking/localization.
CitationTickle, J. A., Jenkins, S. I., Polyark, B., Pickard, M. R., & Chari, D. M. (2016). Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance. Nanomedicine, 11(4), 345-358. http://dx.doi.org/10.2217/nnm.15.202
SponsorsBBSRC, UK; National Heart, Lung and Blood Institute and Drexel University College of Medicine Clinical & Translational Research Institute, CTRIUSA Award Number R01HL107771; EPSRC E-TERM Landscape Fellowship (EP/I017801/1)
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