Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response
AuthorsWaugh, David G.
Smith, Graham C.
AffiliationUniversity of Chester ; University of Chester ; University of Chester ; Queen's University, Belfast ; University of Lincoln ; Hong Kong Polytechnic University ; University of Chester
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AbstractMesenchymal stem cells (MSCs) are known to play important roles in development, post-natal growth, repair, and regeneration of mesenchymal tissues. What is more, surface treatments are widely reported to affect the biomimetic nature of materials. This paper will detail, discuss and compare laser surface treatment of polyamide (Polyamide 6,6), using a 60 W CO2 laser, and NiTi alloy, using a 100 W fiber laser, and the effects of these treatments on mesenchymal stem cell response. The surface morphology and composition of the polyamide and NiTi alloy were studied by scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS), respectively. MSC cell morphology cell counting and viability measurements were done by employing a haemocytometer and MTT colorimetric assay. The success of enhanced adhesion and spreading of the MSCs on each of the laser surface treated samples, when compared to as-received samples, is evidenced in this work.
CitationWaugh, D. G., Lawrence, J., Shukla P., Chan, C. W., Hussain, I., Man, H. C., & Smith, G. C. (2015). Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response. Unpublished conference paper given at the Industrial Laser Applications Symposium at Chesford Grange Conference Centre, Kenilworth, UK, 17-18 March 2015.
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