• Model-directed engineering of “difficult-to-express” monoclonal antibody production by Chinese hamster ovary cells

      Pybus, Leon P.; Dean, Greg; West, Nathan R.; Smith, Andrew; Daramola, Olalekan; Field, Ray; Wilkinson, Stephen J.; James, David C.; University of Sheffield ; BioPharmaceutical Development ; University of Sheffield ; BioPharmaceutical Development ; BioPharmaceutical Development ; BioPharmaceutical Development ; University of Sheffield ; University of Sheffield; ChELSI Institute; Department of Chemical and Biological Engineering; University of Sheffield; Mappin Street Sheffield S1 3JD UK; et al. (Wiley, 2013-11-14)
      Despite improvements in volumetric titer for monoclonal antibody (MAb) production processes using Chinese hamster ovary (CHO) cells, some “difficult-to-express” (DTE) MAbs inexplicably reach much lower process titers.