• In vitro mesenchymal stem cell response to a CO2 laser modified polymeric material

      Waugh, David G.; Hussain, Issam; Lawrence, Jonathan; Smith, Graham C.; Toccaceli, Christina; University of Chester; University of Lincoln (Elsevier, 2016-05-16)
      With an ageing world population it is becoming significantly apparent that there is a need to produce implants and platforms to manipulate stem cell growth on a pharmaceutical scale. This is needed to meet the socio-economic demands of many countries worldwide. This paper details one of the first ever studies in to the manipulation of stem cell growth on CO2 laser surface treated nylon 6,6 highlighting its potential as an inexpensive platform to manipulate stem cell growth on a pharmaceutical scale. Through CO2 laser surface treatment discrete changes to the surfaces were made. That is, the surface roughness of the nylon 6,6 was increased by up to 4.3 µm, the contact angle was modulated by up to 5° and the surface oxygen content increased by up to 1 atom%. Following mesenchymal stem cell growth on the laser treated samples, it was identified that CO2 laser surface treatment gave rise to an enhanced response with an increase in viable cell count of up to 60,000 cells/ml when compared to the as-received sample. The effect of surface parameters modified by the CO2 laser surface treatment on the mesenchymal stem cell response is also discussed along with potential trends that could be identified to govern the mesenchymal stem cell response.
    • Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response

      Waugh, David G.; Lawrence, Jonathan; Shukla, Pratik; Chan, Chi-Wai; Hussain, Issam; Man, Hau-Chung; Smith, Graham C.; University of Chester ; University of Chester ; University of Chester ; Queen's University, Belfast ; University of Lincoln ; Hong Kong Polytechnic University ; University of Chester (2015-03-18)
      Mesenchymal stem cells (MSCs) are known to play important roles in development, post-natal growth, repair, and regeneration of mesenchymal tissues. What is more, surface treatments are widely reported to affect the biomimetic nature of materials. This paper will detail, discuss and compare laser surface treatment of polyamide (Polyamide 6,6), using a 60 W CO2 laser, and NiTi alloy, using a 100 W fiber laser, and the effects of these treatments on mesenchymal stem cell response. The surface morphology and composition of the polyamide and NiTi alloy were studied by scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS), respectively. MSC cell morphology cell counting and viability measurements were done by employing a haemocytometer and MTT colorimetric assay. The success of enhanced adhesion and spreading of the MSCs on each of the laser surface treated samples, when compared to as-received samples, is evidenced in this work.