Platelet-derived growth factor stimulates osteoprotegerin production in osteoblastic cells
Affiliation
Charles Salt Centre, Robert Jones and Agnes Hunt Orthopaedic Hospital NHS Trust in Oswestry / University of Chester ; University of Chester ; Charles Salt Centre, Robert Jones and Agnes Hunt Orthopaedic Hospital NHS Trust in Oswestry ; Charles Salt Centre, Robert Jones and Agnes Hunt Orthopaedic Hospital NHS Trust in OswestryPublication Date
2008-11-20
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This article discusses how osteoprotegerin (OPG) production by osteoblastic cells was stimulated by platelet-derived growth factor (PDGF) in two human osteosarcoma cell lines (MG63, Saos-2), a mouse pre-osteoblastic cell line (MC3T3-E1) and human bone marrow stromal cells (hMSC) by 152%, 197%, 113% and 45% respectively over 24 h. OPG was measured in the cell culture medium by immunoassay. PDGF isoforms AA, BB and AB show similar stimulation of OPG production. Message for OPG was also increased similarly to the increased secretion into the culture medium. Using specific inhibitors of cell signalling the authors demonstrate that PDGF acts through the PDGF receptor, PKC, PI3K, ERK and P38 and not via NF-kB or JNK. The importance of PDGF in fracture healing suggests a role for OPG production in countering bone resorption during the early phase of this process.Citation
Journal of Cellular Physiology, 218(2), 2009, pp. 350-354Publisher
WileyJournal
Journal of Cellular PhysiologyAdditional Links
http://doi.wiley.com/10.1002/jcp.21600Type
ArticleLanguage
enDescription
This article is not available through ChesterRep.ISSN
0021-95411097-4652
Sponsors
The Bone Disease Foundationae974a485f413a2113503eed53cd6c53
10.1002/jcp.21600