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Lysophosphatidic Acid Signalling in Diabetes Mellitus
Asiamah, Prince
Asiamah, Prince
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2023-08
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Abstract
Driven by obesity, the incidence of type 2 diabetes mellitus (T2DM) has escalated, reaching unprecedented levels worldwide. This has led to a change in approach from treatment to prevention or delaying the onset of the disease. While current treatment
of the disease has been successful in increasing patients’ quality of life, it has substantially increased the financial burden on healthcare systems worldwide. In view of this, the NHS, in partnership with Diabetes UK and Public Health England, has
launched a diabetes prevention programme to target people at risk of the disease and provide targeted support to prevent its development. A prelude to this strategy is to identify robust risk factors that will enable the capture of people at increased risk of developing the disease. The phospholipid lysophosphatidic acid (LPA), upregulated during obesity, has been shown to be a potent regulator of glucose homeostasis and insulin secretion in animal models. This thesis is the first to assess circulating levels of LPA in human T2DM patients versus non-DM control. The thesis aims to assess the usefulness of LPA as a biomarker for T2DM screening and has three major objectives: (1) to determine plasma LPA concentration in T2DM patients compared
with non-diabetic controls and assess LPA correlation with current diagnostic markers for T2DM. (2) to determine LPA correlation with pathological markers known to lead to DM complications; (3) to develop an immunofluorescence assay to assess LPA
signalling of glucose homoeostasis using a mammalian cell line. (4) to assess LPA regulations of GLUT4, PKC and α2a adrenergic receptor (α2aAR) using the developed assay.
Citation
Asiamah, P. (2023). Lysophosphatidic acid signalling in Diabetes Mellitus [Unpublished doctoral thesis]. University of Chester.
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University of Chester
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Thesis or dissertation
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en