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Identification of functional Npu DnaE and gp41-1 inteins split in three fragments
Weis, Daniel Palanisamy, Navaneethan Ballestin, Jara Ballestin Öztürk, Mehmet Ali Di Ventura, Barbara
Weis, Daniel
Palanisamy, Navaneethan
Ballestin, Jara Ballestin
Öztürk, Mehmet Ali
Di Ventura, Barbara
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2023-03-06
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Inteins are special proteins that auto-catalytically carry out a protein splicing reaction. Due to their ability to post-translationally modify target proteins in vitro and in vivo, they are used in different applications, ranging from protein purification to the construction of Boolean logic gates. So far inteins have been found to be either encoded by a single gene (contiguous inteins) or by two separate ones (split inteins). Previously, it has been shown that the contiguous Ssp and Rma DnaB inteins and the split Npu DnaE intein could be artificially split in three fragments and retain functionality. Here we report the identification of novel split sites within the N-terminal fragments of the Npu DnaE and gp41-1 split inteins that lead to synthetic functional three-piece versions of these inteins. These variants contribute to the toolkit of three-piece inteins that could be used in biotechnological applications based on highly-fragmented inteins.
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Weis, D., Palanisamy, N., Ballestin, J. B., Öztürk, M. A., & Di Ventura, B. (2023). Identification of functional Npu DnaE and gp41-1 inteins split in three fragments. bioRxiv. https://doi.org/10.1101/2023.01.30.526203
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