Recombination: Multiply infected spleen cells in HIV patients

Hdl Handle:
http://hdl.handle.net/10034/97006
Title:
Recombination: Multiply infected spleen cells in HIV patients
Authors:
Jung, Andreas; Maier, Reinhard; Vartanian, Jean-Pierre; Bocharov, Gennady; Jung, Volker; Fischer, Ulrike; Meese, Eckart; Wain-Hobson, Simon; Meyerhans, Andreas
Abstract:
The genome of the human immunodeficiency virus is highly prone to recombination, although it is not obvious whether recombinants arise infrequently or whether they are constantly being spawned but escape identification because of the massive and rapid turnover of virus particles. Here we use fluorescence in situ hybridization to estimate the number of proviruses harboured by individual splenocytes from two HIV patients, and determine the extent of recombination by sequencing amplified DNA from these cells. We find an average of three or four proviruses per cell and evidence for huge numbers of recombinants and extensive genetic variation. Although this creates problems for phylogenetic analyses, which ignore recombination effects, the intracellular variation may help to broaden immune recognition.
Citation:
Nature, 2002, 418, p. 144
Publisher:
Nature Publishing Group
Journal:
Nature
Publication Date:
11-Jul-2002
URI:
http://hdl.handle.net/10034/97006
DOI:
10.1038/418144a
Additional Links:
http://www.nature.com/nature/index.html
Type:
Article
Language:
en
Description:
This article is not available through ChesterRep.
ISSN:
00280836
Sponsors:
This article was submitted to the RAE2008 for the University of Chester - Allied Health Professions and Studies.
Appears in Collections:
Mathematics

Full metadata record

DC FieldValue Language
dc.contributor.authorJung, Andreasen
dc.contributor.authorMaier, Reinharden
dc.contributor.authorVartanian, Jean-Pierreen
dc.contributor.authorBocharov, Gennadyen
dc.contributor.authorJung, Volkeren
dc.contributor.authorFischer, Ulrikeen
dc.contributor.authorMeese, Eckarten
dc.contributor.authorWain-Hobson, Simonen
dc.contributor.authorMeyerhans, Andreasen
dc.date.accessioned2010-04-21T11:54:48Z-
dc.date.available2010-04-21T11:54:48Z-
dc.date.issued2002-07-11-
dc.identifier.citationNature, 2002, 418, p. 144en
dc.identifier.issn00280836-
dc.identifier.doi10.1038/418144a-
dc.identifier.urihttp://hdl.handle.net/10034/97006-
dc.descriptionThis article is not available through ChesterRep.en
dc.description.abstractThe genome of the human immunodeficiency virus is highly prone to recombination, although it is not obvious whether recombinants arise infrequently or whether they are constantly being spawned but escape identification because of the massive and rapid turnover of virus particles. Here we use fluorescence in situ hybridization to estimate the number of proviruses harboured by individual splenocytes from two HIV patients, and determine the extent of recombination by sequencing amplified DNA from these cells. We find an average of three or four proviruses per cell and evidence for huge numbers of recombinants and extensive genetic variation. Although this creates problems for phylogenetic analyses, which ignore recombination effects, the intracellular variation may help to broaden immune recognition.en
dc.description.sponsorshipThis article was submitted to the RAE2008 for the University of Chester - Allied Health Professions and Studies.en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.urlhttp://www.nature.com/nature/index.htmlen
dc.subjectHIVen
dc.titleRecombination: Multiply infected spleen cells in HIV patientsen
dc.typeArticleen
dc.identifier.journalNatureen
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