Authors
Jung, AndreasMaier, Reinhard
Vartanian, Jean-Pierre
Bocharov, Gennady
Jung, Volker
Fischer, Ulrike
Meese, Eckart
Wain-Hobson, Simon
Meyerhans, Andreas
Publication Date
2002-07-11
Metadata
Show full item recordAbstract
The genome of the human immunodeficiency virus is highly prone to recombination, although it is not obvious whether recombinants arise infrequently or whether they are constantly being spawned but escape identification because of the massive and rapid turnover of virus particles. Here we use fluorescence in situ hybridization to estimate the number of proviruses harboured by individual splenocytes from two HIV patients, and determine the extent of recombination by sequencing amplified DNA from these cells. We find an average of three or four proviruses per cell and evidence for huge numbers of recombinants and extensive genetic variation. Although this creates problems for phylogenetic analyses, which ignore recombination effects, the intracellular variation may help to broaden immune recognition.Citation
Nature, 2002, 418, p. 144Publisher
Nature Publishing GroupJournal
NatureDOI
10.1038/418144aAdditional Links
http://www.nature.com/nature/index.htmlType
ArticleLanguage
enDescription
This article is not available through ChesterRep.ISSN
00280836Sponsors
This article was submitted to the RAE2008 for the University of Chester - Allied Health Professions and Studies.ae974a485f413a2113503eed53cd6c53
10.1038/418144a