Hdl Handle:
http://hdl.handle.net/10034/96635
Title:
Minichromosome maintenance protein in myxofibrosarcoma
Authors:
Sington, James D.; Freeman, Alex; Morris, Lesley S.; Vowler, Sarah L.; Arch, Barbara N.; Fisher, Cyril; Coleman, Nicholas
Abstract:
Histopathological assessment of myxofibrosarcoma may be difficult, especially on the basis of a small core biopsy, which enables only a crude evaluation of grade and prognosis. Hypothesis - that determination of cell cycle state may assist in the diagnostic assessment of myxofibrosarcoma. 51 cases of high-grade (n=20), intermediate-grade (n=21), and low-grade (n=10) myxofibrosarcomas were studied, as well as nine cases of benign myxoma. Cell cycle state within tumors was determined by immunostaining for the recently described marker minichromosome maintenance protein 2 (MCM2), together with Ki67. Labelling indices for both markers were correlated with tumor grade, mitotic index, and time to first recurrence. The MCM2 labelling indices were significantly higher than the Ki-67 labelling indices. Both indices showed a significant correlation with the mitotic index and both showed significant increases with increasing grade of myxofibrosarcoma. The MCM2 labelling index (but not the Ki67 labelling index) showed a significant inverse exponential correlation with the time to first recurrence. Myxoid and cellular areas showed no difference in the MCM2 and Ki-67 labeling index, suggesting that clinically useful information could be obtained from any component of a myxofibrosarcoma sampled in a needle biopsy and/or cytological specimen. We therefore suggest that assessment of cell cycle state may be a useful diagnostic adjunct in the histopathological assessment of myxofibrosarcoma, by enabling more accurate determination of grade and prediction of outcome.
Affiliation:
University of Cambridge ; Royal Marsden Hospital (Fisher)
Citation:
Modern Pathology, 17(2), 2004, pp. 235-240
Publisher:
Nature
Journal:
Modern Pathology
Publication Date:
2004
URI:
http://hdl.handle.net/10034/96635
DOI:
10.1038/modpathol.3800044
Additional Links:
http://www.nature.com/modpathol/index.html
Type:
Article
Language:
en
Description:
This article is not available from CheserRep. The full-text can be accessed at http://www.nature.com/modpathol/journal/v17/n2/full/3800044a.html
ISSN:
0893-3952; 1530-0285
Sponsors:
This article was submitted to the RAE2008 for the University of Chester - Allied Health Professions and Studies.
Appears in Collections:
Biological Sciences

Full metadata record

DC FieldValue Language
dc.contributor.authorSington, James D.en
dc.contributor.authorFreeman, Alexen
dc.contributor.authorMorris, Lesley S.en
dc.contributor.authorVowler, Sarah L.en
dc.contributor.authorArch, Barbara N.en
dc.contributor.authorFisher, Cyrilen
dc.contributor.authorColeman, Nicholasen
dc.date.accessioned2010-04-16T08:19:11Zen
dc.date.available2010-04-16T08:19:11Zen
dc.date.issued2004en
dc.identifier.citationModern Pathology, 17(2), 2004, pp. 235-240en
dc.identifier.issn0893-3952en
dc.identifier.issn1530-0285en
dc.identifier.doi10.1038/modpathol.3800044en
dc.identifier.urihttp://hdl.handle.net/10034/96635en
dc.descriptionThis article is not available from CheserRep. The full-text can be accessed at http://www.nature.com/modpathol/journal/v17/n2/full/3800044a.htmlen
dc.description.abstractHistopathological assessment of myxofibrosarcoma may be difficult, especially on the basis of a small core biopsy, which enables only a crude evaluation of grade and prognosis. Hypothesis - that determination of cell cycle state may assist in the diagnostic assessment of myxofibrosarcoma. 51 cases of high-grade (n=20), intermediate-grade (n=21), and low-grade (n=10) myxofibrosarcomas were studied, as well as nine cases of benign myxoma. Cell cycle state within tumors was determined by immunostaining for the recently described marker minichromosome maintenance protein 2 (MCM2), together with Ki67. Labelling indices for both markers were correlated with tumor grade, mitotic index, and time to first recurrence. The MCM2 labelling indices were significantly higher than the Ki-67 labelling indices. Both indices showed a significant correlation with the mitotic index and both showed significant increases with increasing grade of myxofibrosarcoma. The MCM2 labelling index (but not the Ki67 labelling index) showed a significant inverse exponential correlation with the time to first recurrence. Myxoid and cellular areas showed no difference in the MCM2 and Ki-67 labeling index, suggesting that clinically useful information could be obtained from any component of a myxofibrosarcoma sampled in a needle biopsy and/or cytological specimen. We therefore suggest that assessment of cell cycle state may be a useful diagnostic adjunct in the histopathological assessment of myxofibrosarcoma, by enabling more accurate determination of grade and prediction of outcome.en
dc.description.sponsorshipThis article was submitted to the RAE2008 for the University of Chester - Allied Health Professions and Studies.en
dc.language.isoenen
dc.publisherNatureen
dc.relation.urlhttp://www.nature.com/modpathol/index.htmlen
dc.subjectmyxofibrosarcomaen
dc.subjectrecurrenceen
dc.subjectprognosisen
dc.subjectMCM2en
dc.subjectKi67en
dc.titleMinichromosome maintenance protein in myxofibrosarcomaen
dc.typeArticleen
dc.contributor.departmentUniversity of Cambridge ; Royal Marsden Hospital (Fisher)en
dc.identifier.journalModern Pathologyen
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