Use of pulsed-field gel electrophoresis and flagellin gene typing in identifying clonal groups of campylobacter jejuni and campylobacter coli in farm and clinical environments

Hdl Handle:
http://hdl.handle.net/10034/95155
Title:
Use of pulsed-field gel electrophoresis and flagellin gene typing in identifying clonal groups of campylobacter jejuni and campylobacter coli in farm and clinical environments
Authors:
Fitzgerald, Collette; Stanley, Karen; Andrew, Sarah M.; Jones, Keith
Abstract:
Although campylobacters have been isolated from a wide range of animal hosts, the association between campylobacters isolated from humans and animals in the farm environment is unclear. Flagellin gene typing and pulsed-field gel electrophoresis (PFGE) was used to investigate the genetic diversity among isolates from animals (cattle, sheep, and turkey) in farm environments and sporadic cases of campylobacteriosis in the same geographical area. Forty-eight combined fla types were seen among the 315 Campylobacter isolates studied. Six were found in isolates from all four hosts and represented 50% of the total number of isolates. Seventy-one different SmaI PFGE macrorestriction profiles (mrps) were observed, with 86% of isolates assigned to one of 29 different mrps. Fifty-seven isolates from diverse hosts, times, and sources had an identical SmaI mrp and combined fla type. Conversely, a number of genotypes were unique to a particular host. Molecular evidence is provided which suggests a link between campylobacters in the farm environment with those causing disease in the community.
Affiliation:
Lancaster University
Citation:
Applied and Environmental Microbiology, 2001, 67(4), pp. 1429-1436
Publisher:
American Society for Microbiology
Journal:
Applied and Environmental Microbiology
Publication Date:
Apr-2001
URI:
http://hdl.handle.net/10034/95155
DOI:
10.1128/AEM.67.4.1429-1436.2001
Additional Links:
http://aem.asm.org
Type:
Article
Language:
en
Description:
This article is not available through ChesterRep. The full-text is available at http://aem.asm.org/cgi/content/full/67/4/1429
ISSN:
0099-2240
Sponsors:
This article was submitted to the RAE2008 for the University of Chester - Allied Health Professions and Studies.
Appears in Collections:
Biological Sciences

Full metadata record

DC FieldValue Language
dc.contributor.authorFitzgerald, Colletteen
dc.contributor.authorStanley, Karenen
dc.contributor.authorAndrew, Sarah M.en
dc.contributor.authorJones, Keithen
dc.date.accessioned2010-03-29T11:08:56Zen
dc.date.available2010-03-29T11:08:56Zen
dc.date.issued2001-04en
dc.identifier.citationApplied and Environmental Microbiology, 2001, 67(4), pp. 1429-1436en
dc.identifier.issn0099-2240en
dc.identifier.doi10.1128/AEM.67.4.1429-1436.2001en
dc.identifier.urihttp://hdl.handle.net/10034/95155en
dc.descriptionThis article is not available through ChesterRep. The full-text is available at http://aem.asm.org/cgi/content/full/67/4/1429en
dc.description.abstractAlthough campylobacters have been isolated from a wide range of animal hosts, the association between campylobacters isolated from humans and animals in the farm environment is unclear. Flagellin gene typing and pulsed-field gel electrophoresis (PFGE) was used to investigate the genetic diversity among isolates from animals (cattle, sheep, and turkey) in farm environments and sporadic cases of campylobacteriosis in the same geographical area. Forty-eight combined fla types were seen among the 315 Campylobacter isolates studied. Six were found in isolates from all four hosts and represented 50% of the total number of isolates. Seventy-one different SmaI PFGE macrorestriction profiles (mrps) were observed, with 86% of isolates assigned to one of 29 different mrps. Fifty-seven isolates from diverse hosts, times, and sources had an identical SmaI mrp and combined fla type. Conversely, a number of genotypes were unique to a particular host. Molecular evidence is provided which suggests a link between campylobacters in the farm environment with those causing disease in the community.en
dc.description.sponsorshipThis article was submitted to the RAE2008 for the University of Chester - Allied Health Professions and Studies.en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.urlhttp://aem.asm.orgen
dc.subjectcampylobactersen
dc.subjectfarm animalsen
dc.subjectdiseasesen
dc.titleUse of pulsed-field gel electrophoresis and flagellin gene typing in identifying clonal groups of campylobacter jejuni and campylobacter coli in farm and clinical environmentsen
dc.typeArticleen
dc.contributor.departmentLancaster Universityen
dc.identifier.journalApplied and Environmental Microbiologyen
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