Using sub-micron silver-nanoparticle based films to counter biofilm formation by Gram-negative bacteria

Hdl Handle:
http://hdl.handle.net/10034/620901
Title:
Using sub-micron silver-nanoparticle based films to counter biofilm formation by Gram-negative bacteria
Authors:
Gillett, Alice R.; Baxter, S. N.; Hodgson, Simon D.; Smith, Graham C.; Thomas, P. J.
Abstract:
Composite films comprised of silver nanoparticles (AgNPs) grown using a low-cost straightforward chemical bath based method have been deposited on glass microscope slides to investigate their potential as a sacrificial antibacterial coating. The as-deposited films have been characterised using scanning electron microscopy (SEM) and optical profilometry. These suggested that the films were relatively uniform in coverage. Chemical composition of the AgNP films has been studied by using x-ray photoelectron spectroscopy (XPS). The XPS analysis indicated that the Ag was in a metallic form able to sustain plasmon behaviour, and that low levels of residual nanoparticle precursors were present. Particle size was characterised using transmission electron microscopy (TEM) which showed an average particle size of 10.6 nm. The effectiveness of the films as an antibacterial coating was tested against Escherichia coli. The AgNP film was determined to be effective in the killing of E.coli cells over a 24 hour period when compared to equivalent samples that contained no silver. Of particular note was that only minimal bacterial growth was detected over the first 12 hours of testing, up to 78.6 times less than the control samples, suggesting the film is very efficient at slowing initial biofilm formation. The use of AgNP based films that have been synthesised using a novel low-cost, low-temperature and highly upscalable method is demonstrated as a promising solution for the deployment of silver as an effective sacrificial antimicrobial coating to counter the formation of potentially hazardous Gram negative biofilms.
Affiliation:
University of Chester; Bangor University
Citation:
Gillett, A. R., Baxter, S. N., Hodgson, S. D., Smith, G. C., & Thomas, P. J. (2018). Using sub-micron silver-nanoparticle based films to counter biofilm formation by Gram-negative bacteria. Applied Surface Science, 442, 288-297. https://doi.org/10.1016/j.apsusc.2018.02.116
Publisher:
Elsevier
Journal:
Applied Surface Science
Publication Date:
16-Feb-2018
URI:
http://hdl.handle.net/10034/620901
DOI:
10.1016/j.apsusc.2018.02.116
Additional Links:
https://www.sciencedirect.com/science/article/pii/S0169433218304604
Type:
Article
Language:
en
ISSN:
0169-4332
Appears in Collections:
Natural Sciences

Full metadata record

DC FieldValue Language
dc.contributor.authorGillett, Alice R.en
dc.contributor.authorBaxter, S. N.en
dc.contributor.authorHodgson, Simon D.en
dc.contributor.authorSmith, Graham C.en
dc.contributor.authorThomas, P. J.en
dc.date.accessioned2018-03-05T14:50:35Z-
dc.date.available2018-03-05T14:50:35Z-
dc.date.issued2018-02-16-
dc.identifier.citationGillett, A. R., Baxter, S. N., Hodgson, S. D., Smith, G. C., & Thomas, P. J. (2018). Using sub-micron silver-nanoparticle based films to counter biofilm formation by Gram-negative bacteria. Applied Surface Science, 442, 288-297. https://doi.org/10.1016/j.apsusc.2018.02.116en
dc.identifier.issn0169-4332-
dc.identifier.doi10.1016/j.apsusc.2018.02.116-
dc.identifier.urihttp://hdl.handle.net/10034/620901-
dc.description.abstractComposite films comprised of silver nanoparticles (AgNPs) grown using a low-cost straightforward chemical bath based method have been deposited on glass microscope slides to investigate their potential as a sacrificial antibacterial coating. The as-deposited films have been characterised using scanning electron microscopy (SEM) and optical profilometry. These suggested that the films were relatively uniform in coverage. Chemical composition of the AgNP films has been studied by using x-ray photoelectron spectroscopy (XPS). The XPS analysis indicated that the Ag was in a metallic form able to sustain plasmon behaviour, and that low levels of residual nanoparticle precursors were present. Particle size was characterised using transmission electron microscopy (TEM) which showed an average particle size of 10.6 nm. The effectiveness of the films as an antibacterial coating was tested against Escherichia coli. The AgNP film was determined to be effective in the killing of E.coli cells over a 24 hour period when compared to equivalent samples that contained no silver. Of particular note was that only minimal bacterial growth was detected over the first 12 hours of testing, up to 78.6 times less than the control samples, suggesting the film is very efficient at slowing initial biofilm formation. The use of AgNP based films that have been synthesised using a novel low-cost, low-temperature and highly upscalable method is demonstrated as a promising solution for the deployment of silver as an effective sacrificial antimicrobial coating to counter the formation of potentially hazardous Gram negative biofilms.en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttps://www.sciencedirect.com/science/article/pii/S0169433218304604en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectSilver nanoparticleen
dc.subjectAntibacterialen
dc.subjectXPSen
dc.subjectTEMen
dc.subjectBiotechnologyen
dc.subjectE. Colien
dc.titleUsing sub-micron silver-nanoparticle based films to counter biofilm formation by Gram-negative bacteriaen
dc.typeArticleen
dc.contributor.departmentUniversity of Chester; Bangor Universityen
dc.identifier.journalApplied Surface Scienceen
dc.date.accepted2018-02-11-
or.grant.openaccessYesen
rioxxterms.funderUnfundeden
rioxxterms.identifier.projectUnfundeden
rioxxterms.versionAMen
rioxxterms.licenseref.startdate2020-02-16-
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