Retroviral insertional mutagenesis implicates E3 ubiquitin ligase RNF168 in the control of cell proliferation and survival

Hdl Handle:
http://hdl.handle.net/10034/620626
Title:
Retroviral insertional mutagenesis implicates E3 ubiquitin ligase RNF168 in the control of cell proliferation and survival
Authors:
Kizilors, Aytug; Pickard, Mark R.; Schulte, Cathleen E.; Yacqub-Usman, Kiren; McCarthy, Nicola J.; Gan, Shu-Uin; Darling, David; Gäken, Joop; Williams, Gwyn T.; Farzaneh, Farzin
Abstract:
The E3 ubiquitin ligase RNF168 is a ring finger protein that has previously been identified to play an important regulatory role in the repair of double-strand DNA breaks. In the present study, an unbiased forward genetics functional screen in mouse granulocyte/ macrophage progenitor cell line FDCP1 has identified E3 ubiquitin ligase RNF168 as a key regulator of cell survival and proliferation. Our data indicate that RNF168 is an important component of the mechanisms controlling cell fate, not only in human and mouse haematopoietic growth factor-dependent cells, but also in the human breast epithelial cell line MCF-7. These observations therefore suggest that RNF168 provides a connection to key pathways controlling cell fate, potentially through interaction with PML nuclear bodies and/or epigenetic control of gene expression. Our study is the first to demonstrate a critical role for RNF168 in the in the mechanisms regulating cell proliferation and survival, in addition to its well-established role in DNA repair.
Affiliation:
Kings College London; Keele University; University of Chester; University of Nottingham; National University of Singapore
Citation:
Kizilors, A., et al. (2017). Retroviral insertional mutagenesis implicates E3 ubiquitin ligase RNF168 in the control of cell proliferation and survival. Bioscience Reports, 37(4), BSR20170843. DOI: 10.1042/BSR20170843
Publisher:
Portland Press
Journal:
Bioscience reports
Publication Date:
14-Aug-2017
URI:
http://hdl.handle.net/10034/620626
DOI:
10.1042/BSR20170843
Additional Links:
http://www.bioscirep.org/content/37/4/BSR20170843
Type:
Article
Language:
en
EISSN:
1573-4935
Appears in Collections:
Chester Medical School

Full metadata record

DC FieldValue Language
dc.contributor.authorKizilors, Aytugen
dc.contributor.authorPickard, Mark R.en
dc.contributor.authorSchulte, Cathleen E.en
dc.contributor.authorYacqub-Usman, Kirenen
dc.contributor.authorMcCarthy, Nicola J.en
dc.contributor.authorGan, Shu-Uinen
dc.contributor.authorDarling, Daviden
dc.contributor.authorGäken, Joopen
dc.contributor.authorWilliams, Gwyn T.en
dc.contributor.authorFarzaneh, Farzinen
dc.date.accessioned2017-09-25T13:00:55Z-
dc.date.available2017-09-25T13:00:55Z-
dc.date.issued2017-08-14-
dc.identifier.citationKizilors, A., et al. (2017). Retroviral insertional mutagenesis implicates E3 ubiquitin ligase RNF168 in the control of cell proliferation and survival. Bioscience Reports, 37(4), BSR20170843. DOI: 10.1042/BSR20170843en
dc.identifier.doi10.1042/BSR20170843-
dc.identifier.urihttp://hdl.handle.net/10034/620626-
dc.description.abstractThe E3 ubiquitin ligase RNF168 is a ring finger protein that has previously been identified to play an important regulatory role in the repair of double-strand DNA breaks. In the present study, an unbiased forward genetics functional screen in mouse granulocyte/ macrophage progenitor cell line FDCP1 has identified E3 ubiquitin ligase RNF168 as a key regulator of cell survival and proliferation. Our data indicate that RNF168 is an important component of the mechanisms controlling cell fate, not only in human and mouse haematopoietic growth factor-dependent cells, but also in the human breast epithelial cell line MCF-7. These observations therefore suggest that RNF168 provides a connection to key pathways controlling cell fate, potentially through interaction with PML nuclear bodies and/or epigenetic control of gene expression. Our study is the first to demonstrate a critical role for RNF168 in the in the mechanisms regulating cell proliferation and survival, in addition to its well-established role in DNA repair.en
dc.language.isoenen
dc.publisherPortland Pressen
dc.relation.urlhttp://www.bioscirep.org/content/37/4/BSR20170843en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectRetroviral Insertional Mutagenesisen
dc.subjectUbiquitinen
dc.subjectApoptosisen
dc.subjectCell proliferationen
dc.subjectCell survivalen
dc.titleRetroviral insertional mutagenesis implicates E3 ubiquitin ligase RNF168 in the control of cell proliferation and survivalen
dc.typeArticleen
dc.identifier.eissn1573-4935-
dc.contributor.departmentKings College London; Keele University; University of Chester; University of Nottingham; National University of Singaporeen
dc.identifier.journalBioscience reportsen
dc.date.accepted2017-07-27-
or.grant.openaccessYesen
rioxxterms.funderBloodwise; Breast Cancer Now; Rosetrees Trust; National Institute for Health Research (NIHR) Biomedical Research Centre (BRC)en
rioxxterms.identifier.projectNon-RCUK externally funded research to Kings College London & Keele Universityen
rioxxterms.versionAMen
rioxxterms.licenseref.startdate2017-08-14-
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