Retroviral insertional mutagenesis implicates E3 ubiquitin ligase RNF168 in the control of cell proliferation and survival
Authors
Kizilors, AytugPickard, Mark R.
Schulte, Cathleen E.
Yacqub-Usman, Kiren
McCarthy, Nicola J.
Gan, Shu-Uin
Darling, David
Gäken, Joop
Williams, Gwyn T.
Farzaneh, Farzin
Affiliation
Kings College London; Keele University; University of Chester; University of Nottingham; National University of SingaporePublication Date
2017-08-14
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The E3 ubiquitin ligase RNF168 is a ring finger protein that has previously been identified to play an important regulatory role in the repair of double-strand DNA breaks. In the present study, an unbiased forward genetics functional screen in mouse granulocyte/ macrophage progenitor cell line FDCP1 has identified E3 ubiquitin ligase RNF168 as a key regulator of cell survival and proliferation. Our data indicate that RNF168 is an important component of the mechanisms controlling cell fate, not only in human and mouse haematopoietic growth factor-dependent cells, but also in the human breast epithelial cell line MCF-7. These observations therefore suggest that RNF168 provides a connection to key pathways controlling cell fate, potentially through interaction with PML nuclear bodies and/or epigenetic control of gene expression. Our study is the first to demonstrate a critical role for RNF168 in the in the mechanisms regulating cell proliferation and survival, in addition to its well-established role in DNA repair.Citation
Kizilors, A., Pickard, M. R., Schulte, C. E., Yacqub-Usman, K., McCarthy, N. J., Gan, S-U., Darling, D., Gäken, J., Williams, G. T., & Farzaneh, F. (2017). Retroviral insertional mutagenesis implicates E3 ubiquitin ligase RNF168 in the control of cell proliferation and survival. Bioscience Reports, 37(4), BSR20170843. https://doi.org/10.1042/BSR20170843Publisher
Portland PressJournal
Bioscience ReportsAdditional Links
http://www.bioscirep.org/content/37/4/BSR20170843Type
ArticleLanguage
enEISSN
1573-4935ae974a485f413a2113503eed53cd6c53
10.1042/BSR20170843
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/