Magnetic nanoparticle-mediated gene delivery to two- and three-dimensional neural stem cell cultures: magnet-assisted transfection and multifection approaches to enhance outcomes

Hdl Handle:
http://hdl.handle.net/10034/620474
Title:
Magnetic nanoparticle-mediated gene delivery to two- and three-dimensional neural stem cell cultures: magnet-assisted transfection and multifection approaches to enhance outcomes
Authors:
Pickard, Mark R.; Adams, Christopher F.; Chari, Divya M.
Abstract:
Neural stem cells (NSCs) have high translational potential in transplantation therapies for neural repair. Enhancement of their therapeutic capacity by genetic engineering is an important goal for regenerative neurology. Magnetic nanoparticles (MNPs) are major non-viral vectors for safe bioengineering of NSCs, offering critical translational benefits over viral vectors, including safety, scalability, and ease of use. This unit describes protocols for the production of suspension (neurosphere) and adherent (monolayer) murine NSC cultures. Genetic engineering of NSCs with MNPs and the application of 'magnetofection' (magnetic fields) or 'multifection' (repeat transfection) approaches to enhance gene delivery are described. Magnetofection of monolayer cultures achieves optimal transfection, but neurospheres offer key advantages for neural graft survival post-transplantation. A protocol is presented which allows the advantageous features of each approach to be combined into a single procedure for transplantation. The adaptation of these protocols for other MNP preparations is considered, with emphasis on the evaluation of procedural safety.
Affiliation:
University of Chester; Keele University
Citation:
Pickard, M. R., Adams, C. F., & Chari, D. M. (2017). Magnetic nanoparticle-mediated gene delivery to two- and three-dimensional neural stem cell cultures: magnet-assisted transfection and multifection approaches to enhance outcomes. Current Protocols in Stem Cell Biology, 40:2D.19.1-2D.19.16. DOI: 10.1002/cpsc.23
Publisher:
Wiley
Journal:
Current Protocols in Stem Cell Biology
Publication Date:
2-Feb-2017
URI:
http://hdl.handle.net/10034/620474
DOI:
10.1002/cpsc.23
Additional Links:
http://onlinelibrary.wiley.com/doi/10.1002/cpsc.23/abstract
Type:
Book chapter
Language:
en
Description:
This is the peer reviewed version of the following article: Pickard, M. R., Adams, C. F., & Chari, D. M. (2017). Magnetic nanoparticle-mediated gene delivery to two- and three-dimensional neural stem cell cultures: magnet-assisted transfection and multifection approaches to enhance outcomes. Current Protocols in Stem Cell Biology, 40:2D.19.1-2D.19.16. DOI: 10.1002/cpsc.23, which has been published in final form at http://dx.doi.org/10.1002/cpsc.23. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving
ISBN:
9780470151808
Appears in Collections:
Institute of Medicine

Full metadata record

DC FieldValue Language
dc.contributor.authorPickard, Mark R.en
dc.contributor.authorAdams, Christopher F.en
dc.contributor.authorChari, Divya M.en
dc.date.accessioned2017-04-07T11:17:29Z-
dc.date.available2017-04-07T11:17:29Z-
dc.date.issued2017-02-02-
dc.identifier.citationPickard, M. R., Adams, C. F., & Chari, D. M. (2017). Magnetic nanoparticle-mediated gene delivery to two- and three-dimensional neural stem cell cultures: magnet-assisted transfection and multifection approaches to enhance outcomes. Current Protocols in Stem Cell Biology, 40:2D.19.1-2D.19.16. DOI: 10.1002/cpsc.23en
dc.identifier.isbn9780470151808-
dc.identifier.doi10.1002/cpsc.23-
dc.identifier.urihttp://hdl.handle.net/10034/620474-
dc.descriptionThis is the peer reviewed version of the following article: Pickard, M. R., Adams, C. F., & Chari, D. M. (2017). Magnetic nanoparticle-mediated gene delivery to two- and three-dimensional neural stem cell cultures: magnet-assisted transfection and multifection approaches to enhance outcomes. Current Protocols in Stem Cell Biology, 40:2D.19.1-2D.19.16. DOI: 10.1002/cpsc.23, which has been published in final form at http://dx.doi.org/10.1002/cpsc.23. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archivingen
dc.description.abstractNeural stem cells (NSCs) have high translational potential in transplantation therapies for neural repair. Enhancement of their therapeutic capacity by genetic engineering is an important goal for regenerative neurology. Magnetic nanoparticles (MNPs) are major non-viral vectors for safe bioengineering of NSCs, offering critical translational benefits over viral vectors, including safety, scalability, and ease of use. This unit describes protocols for the production of suspension (neurosphere) and adherent (monolayer) murine NSC cultures. Genetic engineering of NSCs with MNPs and the application of 'magnetofection' (magnetic fields) or 'multifection' (repeat transfection) approaches to enhance gene delivery are described. Magnetofection of monolayer cultures achieves optimal transfection, but neurospheres offer key advantages for neural graft survival post-transplantation. A protocol is presented which allows the advantageous features of each approach to be combined into a single procedure for transplantation. The adaptation of these protocols for other MNP preparations is considered, with emphasis on the evaluation of procedural safety.en
dc.language.isoenen
dc.publisherWileyen
dc.relation.urlhttp://onlinelibrary.wiley.com/doi/10.1002/cpsc.23/abstracten
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectMagnetic nanoparticlesen
dc.subjectNeural stem cellsen
dc.subjectGene deliveryen
dc.subjectMagnetofectionen
dc.subjectTransplantationen
dc.titleMagnetic nanoparticle-mediated gene delivery to two- and three-dimensional neural stem cell cultures: magnet-assisted transfection and multifection approaches to enhance outcomesen
dc.typeBook chapteren
dc.contributor.departmentUniversity of Chester; Keele Universityen
dc.identifier.journalCurrent Protocols in Stem Cell Biologyen
dc.date.accepted2016-11-02-
or.grant.openaccessYesen
rioxxterms.funderUnfundeden
rioxxterms.identifier.projectUnfundeden
rioxxterms.versionAMen
rioxxterms.licenseref.startdate2217-02-02-
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