Preparation, Characterisation and Measurement of the in vitro Cytotoxicity of Mesoporous Silica Nanoparticles Loaded with Cytotoxic Pt(II) Oxadiazoline Complexes

Hdl Handle:
http://hdl.handle.net/10034/606955
Title:
Preparation, Characterisation and Measurement of the in vitro Cytotoxicity of Mesoporous Silica Nanoparticles Loaded with Cytotoxic Pt(II) Oxadiazoline Complexes
Authors:
Wagner, Gabriele; Herrmann, Rudolf
Abstract:
Cytotoxic platinum compounds play a major role in the chemotherapy of a large number of human cancers. However, due to the severe side effects for the patient and other problems associated with their use, there is a need for the development of more efficient drugs and new methods for their selective delivery to the tumours. One way to achieve the latter could be in the use of nanoparticular carrier materials that can adsorb or chemically bind the drug. In the cell, the drug is supposed to be slowly released, either by physical desorption or by dissolution of the particle framework. Ideally, the cytotoxic properties of the platinum drug unfold only then, in the cancer cell and over a longer period of time due to the gradual release. In this paper, we report on our first steps in this direction. The binding properties of a series of cytotoxic Pt(II) oxadiazoline compounds to mesoporous silica particles has been studied by NMR and UV/vis spectroscopy. High loadings were achieved when the Pt(II) compound was relatively polar, and has been dissolved in a relatively unpolar solvent before the silica was added. Typically, 6-10 hours were required for complete equilibration, suggesting the adsorption did not only occur to the outer surface but also to the interior of the pores. The untreated and Pt(II) loaded particles were characterised by C,H,N combustion analysis, BET/BJH nitrogen sorption, electron microscopy (REM and TEM) and EDX. With the latter methods we were able to demonstrate the homogenous distribution of the Pt(II) compound on and in the silica particles, and no Pt(II) bulk precipitate had formed. The in vitro cytotoxicity in a human cancer cell line (HeLa) has been determined for one of the new platinum compounds adsorbed to mesoporous silica particles of different size, and compared with the corresponding compound in solution. The IC50 data are similar in all cases, suggesting that the release of the Pt(II) compound was relatively fast and possibly occurred before the particles reached the cells. Overall, the platinum drug is chemically stable on silica and retained its activity upon prolonged storage.
Affiliation:
Department of Natural Sciences, University of Chester, Thornton Science Park, Pool Lane, Ince, Chester, CH2 4NU, UK, and Institute of Physics, University of Augsburg, Universitätsstr. 1, D-86135 Augsburg, Germany.
Citation:
Wagner, G., & Herrmann, R. (2016). Preparation, characterisation, and measurement of the in vitro cytotoxicity of mesoporous silica nanoparticles loaded with cytotoxic Pt(II) oxadiazoline complexes. World Academy of Science, Engineering and Technology, International Science Index 111, International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering, 10(3), 93-98.
Publisher:
World Academy of Science, Engineering and Technology
Journal:
International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering
Publication Date:
7-Mar-2016
URI:
http://hdl.handle.net/10034/606955
Additional Links:
http://waset.org/Publication/preparation-characterisation-and-measurement-of-the-in-vitro-cytotoxicity-of-mesoporous-silica-nanoparticles-loaded-with-cytotoxic-pt-ii-oxadiazoline-complexes/10003959
Type:
Article
Language:
en
Appears in Collections:
Natural Sciences

Full metadata record

DC FieldValue Language
dc.contributor.authorWagner, Gabrieleen
dc.contributor.authorHerrmann, Rudolfen
dc.date.accessioned2016-04-25T13:33:21Zen
dc.date.available2016-04-25T13:33:21Zen
dc.date.issued2016-03-07en
dc.identifier.citationWagner, G., & Herrmann, R. (2016). Preparation, characterisation, and measurement of the in vitro cytotoxicity of mesoporous silica nanoparticles loaded with cytotoxic Pt(II) oxadiazoline complexes. World Academy of Science, Engineering and Technology, International Science Index 111, International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering, 10(3), 93-98.en
dc.identifier.otherNAen
dc.identifier.urihttp://hdl.handle.net/10034/606955en
dc.description.abstractCytotoxic platinum compounds play a major role in the chemotherapy of a large number of human cancers. However, due to the severe side effects for the patient and other problems associated with their use, there is a need for the development of more efficient drugs and new methods for their selective delivery to the tumours. One way to achieve the latter could be in the use of nanoparticular carrier materials that can adsorb or chemically bind the drug. In the cell, the drug is supposed to be slowly released, either by physical desorption or by dissolution of the particle framework. Ideally, the cytotoxic properties of the platinum drug unfold only then, in the cancer cell and over a longer period of time due to the gradual release. In this paper, we report on our first steps in this direction. The binding properties of a series of cytotoxic Pt(II) oxadiazoline compounds to mesoporous silica particles has been studied by NMR and UV/vis spectroscopy. High loadings were achieved when the Pt(II) compound was relatively polar, and has been dissolved in a relatively unpolar solvent before the silica was added. Typically, 6-10 hours were required for complete equilibration, suggesting the adsorption did not only occur to the outer surface but also to the interior of the pores. The untreated and Pt(II) loaded particles were characterised by C,H,N combustion analysis, BET/BJH nitrogen sorption, electron microscopy (REM and TEM) and EDX. With the latter methods we were able to demonstrate the homogenous distribution of the Pt(II) compound on and in the silica particles, and no Pt(II) bulk precipitate had formed. The in vitro cytotoxicity in a human cancer cell line (HeLa) has been determined for one of the new platinum compounds adsorbed to mesoporous silica particles of different size, and compared with the corresponding compound in solution. The IC50 data are similar in all cases, suggesting that the release of the Pt(II) compound was relatively fast and possibly occurred before the particles reached the cells. Overall, the platinum drug is chemically stable on silica and retained its activity upon prolonged storage.en
dc.language.isoenen
dc.publisherWorld Academy of Science, Engineering and Technologyen
dc.relation.urlhttp://waset.org/Publication/preparation-characterisation-and-measurement-of-the-in-vitro-cytotoxicity-of-mesoporous-silica-nanoparticles-loaded-with-cytotoxic-pt-ii-oxadiazoline-complexes/10003959en
dc.rightsAn error occurred on the license name.*
dc.rights.uriAn error occurred getting the license - uri.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectcytotoxicityen
dc.subjectmesoporous silica particlesen
dc.subjectplatinum compoundsen
dc.titlePreparation, Characterisation and Measurement of the in vitro Cytotoxicity of Mesoporous Silica Nanoparticles Loaded with Cytotoxic Pt(II) Oxadiazoline Complexesen
dc.typeArticleen
dc.contributor.departmentDepartment of Natural Sciences, University of Chester, Thornton Science Park, Pool Lane, Ince, Chester, CH2 4NU, UK, and Institute of Physics, University of Augsburg, Universitätsstr. 1, D-86135 Augsburg, Germany.en
dc.identifier.journalInternational Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineeringen
dc.date.accepted2016-03-07en
or.grant.openaccessYesen
rioxxterms.funderUniversity of Augsburg, University of Ulmen
rioxxterms.identifier.projectunfunded (funded by previous workplace)en
rioxxterms.versionAMen
rioxxterms.licenseref.startdate2016-03-07en
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