Alignment of multiple glial cell populations in 3D nanofiber scaffolds: toward the development of multicellular implantable scaffolds for repair of neural injury

Hdl Handle:
http://hdl.handle.net/10034/606276
Title:
Alignment of multiple glial cell populations in 3D nanofiber scaffolds: toward the development of multicellular implantable scaffolds for repair of neural injury
Authors:
Weightman, Alan P.; Jenkins, Stuart I.; Pickard, Mark R.; Chari, Divya M.; Yang, Ying
Abstract:
Non-neuronal cells of the central nervous system (CNS), termed "neuroglia," play critical roles in neural regeneration; therefore, replacement of glial populations via implantable nanofabricated devices (providing a growth-permissive niche) is a promising strategy to enhance repair. Most constructs developed to date have lacked three-dimensionality, multiple glial populations and control over spatial orientations, limiting their ability to mimic in vivo neurocytoarchitecture. We describe a facile technique to incorporate multiple glial cell populations [astrocytes, oligodendrocyte precursor cells (OPCs) and oligodendrocytes] within a three-dimensional (3D) nanofabricated construct. Highly aligned nanofibers could induce elongation of astrocytes, while OPC survival, elongation and maturation required pre-aligned astrocytes. The potential to scale-up the numbers of constituent nanofiber layers is demonstrated with astrocytes. Such complex implantable constructs with multiple glial sub-populations in defined 3D orientations could represent an effective approach to reconstruct glial circuitry in neural injury sites.
Affiliation:
Keele University, United Kingdom
Citation:
Weightman, A., Jenkins, S., Pickard, m., Chari, D., & Yang, Y. (2014). Alignment of multiple glial cell populations in 3D nanofiber scaffolds: toward the development of multicellular implantable scaffolds for repair of neural injury. Nanomedicine: Nanotechnology, Biology and Medicine, 10(2), 291-95. http://dx.doi.org/10.1016/j.nano.2013.09.001
Publisher:
Elsevier
Journal:
Nanomedicine: Nanotechnology, Biology and Medicine
Publication Date:
Feb-2014
URI:
http://hdl.handle.net/10034/606276
DOI:
10.1016/j.nano.2013.09.001
Additional Links:
http://www.journals.elsevier.com/nanomedicine-nanotechnology-biology-and-medicine
Type:
Article
Language:
en
ISSN:
1549-9634
Appears in Collections:
Institute of Medicine

Full metadata record

DC FieldValue Language
dc.contributor.authorWeightman, Alan P.en
dc.contributor.authorJenkins, Stuart I.en
dc.contributor.authorPickard, Mark R.en
dc.contributor.authorChari, Divya M.en
dc.contributor.authorYang, Yingen
dc.date.accessioned2016-04-21T11:20:40Zen
dc.date.available2016-04-21T11:20:40Zen
dc.date.issued2014-02en
dc.identifier.citationWeightman, A., Jenkins, S., Pickard, m., Chari, D., & Yang, Y. (2014). Alignment of multiple glial cell populations in 3D nanofiber scaffolds: toward the development of multicellular implantable scaffolds for repair of neural injury. Nanomedicine: Nanotechnology, Biology and Medicine, 10(2), 291-95. http://dx.doi.org/10.1016/j.nano.2013.09.001en
dc.identifier.issn1549-9634en
dc.identifier.doi10.1016/j.nano.2013.09.001en
dc.identifier.otherPMID 24090767en
dc.identifier.urihttp://hdl.handle.net/10034/606276en
dc.description.abstractNon-neuronal cells of the central nervous system (CNS), termed "neuroglia," play critical roles in neural regeneration; therefore, replacement of glial populations via implantable nanofabricated devices (providing a growth-permissive niche) is a promising strategy to enhance repair. Most constructs developed to date have lacked three-dimensionality, multiple glial populations and control over spatial orientations, limiting their ability to mimic in vivo neurocytoarchitecture. We describe a facile technique to incorporate multiple glial cell populations [astrocytes, oligodendrocyte precursor cells (OPCs) and oligodendrocytes] within a three-dimensional (3D) nanofabricated construct. Highly aligned nanofibers could induce elongation of astrocytes, while OPC survival, elongation and maturation required pre-aligned astrocytes. The potential to scale-up the numbers of constituent nanofiber layers is demonstrated with astrocytes. Such complex implantable constructs with multiple glial sub-populations in defined 3D orientations could represent an effective approach to reconstruct glial circuitry in neural injury sites.en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttp://www.journals.elsevier.com/nanomedicine-nanotechnology-biology-and-medicineen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subject3D implanten
dc.subjectElectrospinningen
dc.subjectNanofiber scaffoldsen
dc.subjectNeural regenerationen
dc.subjectNeurogliaen
dc.titleAlignment of multiple glial cell populations in 3D nanofiber scaffolds: toward the development of multicellular implantable scaffolds for repair of neural injuryen
dc.typeArticleen
dc.contributor.departmentKeele University, United Kingdomen
dc.identifier.journalNanomedicine: Nanotechnology, Biology and Medicineen
dc.date.accepted2013-09-13en
or.grant.openaccessYesen
rioxxterms.funderEPSRCen
rioxxterms.identifier.projectEPSRC Doctoral Training Center in regenerative medicine (EP/F500491/1)en
rioxxterms.versionAMen
rioxxterms.licenseref.startdate2016-04-21en
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