Influence of Amplitude of Oscillating Magnetic Fields on Magnetic Nanoparticle-Mediated Gene Transfer to Astrocytes

Hdl Handle:
http://hdl.handle.net/10034/605231
Title:
Influence of Amplitude of Oscillating Magnetic Fields on Magnetic Nanoparticle-Mediated Gene Transfer to Astrocytes
Authors:
Tickle, Jacqueline A.; Jenkins, Stuart I.; Pickard, Mark R.; Chari, Divya M.
Abstract:
Functionalized magnetic nanoparticles (MNPs) are emerging as a major nanoplatform for regenerative neurology, particularly as transfection agents for gene delivery. Magnetic assistive technology, particularly the recent innovation of applied oscillating magnetic fields, can significantly enhance MNP-mediated gene transfer to neural cells. While transfection efficiency varies with oscillation frequency in various neural cell types, the influence of oscillation amplitude has not yet been investigated. We have addressed this issue using cortical astrocytes that were transfected using MNPs functionalized with plasmid encoding a reporter protein. Cells were exposed to a range of oscillation amplitudes (100–1000 μm), using a fixed oscillation frequency of 1 Hz. No significant differences were found in the proportions of transfected cells at the amplitudes tested, but GFP-related optical density measurements (indicative of reporter protein expression) were significantly enhanced at 200 μm. Safety data show no amplitude-dependent toxicity. Our data suggest that the amplitude of oscillating magnetic fields influences MNP-mediated transfection, and a tailored combination of amplitude and frequency may further enhance transgene expression. Systematic testing of these parameters in different neural subtypes will enable the development of a database of neuro-magnetofection protocols — an area of nanotechnology research where little information currently exists.
Affiliation:
Keele University, United Kingdom
Citation:
Tickle, J. A., Jenkins, S. I., Pickard, M. R., & Chari, D. M. (2014). Influence of amplitude of oscillating magnetic fields on magnetic nanoparticle-mediated gene transfer to astrocytes. Nano LIFE, 5(1). http://dx.doi.org/10.1142/S1793984414500068
Publisher:
World Scientific
Journal:
Nano LIFE
Publication Date:
7-Aug-2014
URI:
http://hdl.handle.net/10034/605231
DOI:
10.1142/S1793984414500068
Additional Links:
http://www.worldscientific.com/doi/pdf/10.1142/S1793984414500068
Type:
Article
Language:
en
Description:
Electronic version of an article published as Nano LIFE, Volume 5, Issue 1, 2014, Article DOI:10.1142/S1793984414500068 © copyright World Scientific Publishing Company. http://www.worldscientific.com/loi/nl
ISSN:
1793-9844
EISSN:
1793-9852
Appears in Collections:
Institute of Medicine

Full metadata record

DC FieldValue Language
dc.contributor.authorTickle, Jacqueline A.en
dc.contributor.authorJenkins, Stuart I.en
dc.contributor.authorPickard, Mark R.en
dc.contributor.authorChari, Divya M.en
dc.date.accessioned2016-04-14T10:09:30Zen
dc.date.available2016-04-14T10:09:30Zen
dc.date.issued2014-08-07en
dc.identifier.citationTickle, J. A., Jenkins, S. I., Pickard, M. R., & Chari, D. M. (2014). Influence of amplitude of oscillating magnetic fields on magnetic nanoparticle-mediated gene transfer to astrocytes. Nano LIFE, 5(1). http://dx.doi.org/10.1142/S1793984414500068en
dc.identifier.issn1793-9844en
dc.identifier.doi10.1142/S1793984414500068en
dc.identifier.urihttp://hdl.handle.net/10034/605231en
dc.descriptionElectronic version of an article published as Nano LIFE, Volume 5, Issue 1, 2014, Article DOI:10.1142/S1793984414500068 © copyright World Scientific Publishing Company. http://www.worldscientific.com/loi/nlen
dc.description.abstractFunctionalized magnetic nanoparticles (MNPs) are emerging as a major nanoplatform for regenerative neurology, particularly as transfection agents for gene delivery. Magnetic assistive technology, particularly the recent innovation of applied oscillating magnetic fields, can significantly enhance MNP-mediated gene transfer to neural cells. While transfection efficiency varies with oscillation frequency in various neural cell types, the influence of oscillation amplitude has not yet been investigated. We have addressed this issue using cortical astrocytes that were transfected using MNPs functionalized with plasmid encoding a reporter protein. Cells were exposed to a range of oscillation amplitudes (100–1000 μm), using a fixed oscillation frequency of 1 Hz. No significant differences were found in the proportions of transfected cells at the amplitudes tested, but GFP-related optical density measurements (indicative of reporter protein expression) were significantly enhanced at 200 μm. Safety data show no amplitude-dependent toxicity. Our data suggest that the amplitude of oscillating magnetic fields influences MNP-mediated transfection, and a tailored combination of amplitude and frequency may further enhance transgene expression. Systematic testing of these parameters in different neural subtypes will enable the development of a database of neuro-magnetofection protocols — an area of nanotechnology research where little information currently exists.en
dc.language.isoenen
dc.publisherWorld Scientificen
dc.relation.urlhttp://www.worldscientific.com/doi/pdf/10.1142/S1793984414500068en
dc.subjectAstrocytesen
dc.subjectmagnetic nanoparticlesen
dc.subjectmagnetofectionen
dc.subjectnonviral transfectionen
dc.subjectgene deliveryen
dc.titleInfluence of Amplitude of Oscillating Magnetic Fields on Magnetic Nanoparticle-Mediated Gene Transfer to Astrocytesen
dc.typeArticleen
dc.identifier.eissn1793-9852en
dc.contributor.departmentKeele University, United Kingdomen
dc.identifier.journalNano LIFEen
dc.date.accepted2014-06-16en
or.grant.openaccessNoen
rioxxterms.funderxxen
rioxxterms.identifier.projectxxen
rioxxterms.versionNAen
rioxxterms.licenseref.startdate2214-08-07en
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