Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance

Hdl Handle:
http://hdl.handle.net/10034/604463
Title:
Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance
Authors:
Tickle, Jacqueline A.; Jenkins, Stuart I.; Polyak, Boris; Pickard, Mark R.; Chari, Divya M.
Abstract:
AIM: To achieve high and sustained magnetic particle loading in a proliferative and endocytotically active neural transplant population (astrocytes) through tailored magnetite content in polymeric iron oxide particles. MATERIALS & METHODS: MPs of varying magnetite content were applied to primary-derived rat cortical astrocytes ± static/oscillating magnetic fields to assess labeling efficiency and safety. RESULTS: Higher magnetite content particles display high but safe accumulation in astrocytes, with longer-term label retention versus lower/no magnetite content particles. Magnetic fields enhanced loading extent. Dynamic live cell imaging of dividing labeled astrocytes demonstrated that particle distribution into daughter cells is predominantly 'asymmetric'. CONCLUSION: These findings could inform protocols to achieve efficient MP loading into neural transplant cells, with significant implications for post-transplantation tracking/localization.
Affiliation:
Keele University, United Kingdom; Drexel University College of Medicine, Philadelphia, USA
Citation:
Tickle, J. A., Jenkins, S. I., Polyark, B., Pickard, M. R., & Chari, D. M. (2016). Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance. Nanomedicine, 11(4), 345-358. http://dx.doi.org/10.2217/nnm.15.202
Publisher:
Future Medicine
Journal:
Nanomedicine
Publication Date:
Feb-2016
URI:
http://hdl.handle.net/10034/604463
DOI:
10.2217/nnm.15.202
PubMed ID:
26785794
Additional Links:
http://www.futuremedicine.com/doi/10.2217/nnm.15.202
Type:
Article
Language:
en
ISSN:
1743-5889
EISSN:
1748-6963
Sponsors:
BBSRC, UK; National Heart, Lung and Blood Institute and Drexel University College of Medicine Clinical & Translational Research Institute, CTRIUSA Award Number R01HL107771; EPSRC E-TERM Landscape Fellowship (EP/I017801/1)
Appears in Collections:
Institute of Medicine

Full metadata record

DC FieldValue Language
dc.contributor.authorTickle, Jacqueline A.en
dc.contributor.authorJenkins, Stuart I.en
dc.contributor.authorPolyak, Borisen
dc.contributor.authorPickard, Mark R.en
dc.contributor.authorChari, Divya M.en
dc.date.accessioned2016-04-05T15:07:11Zen
dc.date.available2016-04-05T15:07:11Zen
dc.date.issued2016-02en
dc.identifier.citationTickle, J. A., Jenkins, S. I., Polyark, B., Pickard, M. R., & Chari, D. M. (2016). Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance. Nanomedicine, 11(4), 345-358. http://dx.doi.org/10.2217/nnm.15.202en
dc.identifier.issn1743-5889en
dc.identifier.pmid26785794en
dc.identifier.doi10.2217/nnm.15.202en
dc.identifier.urihttp://hdl.handle.net/10034/604463en
dc.description.abstractAIM: To achieve high and sustained magnetic particle loading in a proliferative and endocytotically active neural transplant population (astrocytes) through tailored magnetite content in polymeric iron oxide particles. MATERIALS & METHODS: MPs of varying magnetite content were applied to primary-derived rat cortical astrocytes ± static/oscillating magnetic fields to assess labeling efficiency and safety. RESULTS: Higher magnetite content particles display high but safe accumulation in astrocytes, with longer-term label retention versus lower/no magnetite content particles. Magnetic fields enhanced loading extent. Dynamic live cell imaging of dividing labeled astrocytes demonstrated that particle distribution into daughter cells is predominantly 'asymmetric'. CONCLUSION: These findings could inform protocols to achieve efficient MP loading into neural transplant cells, with significant implications for post-transplantation tracking/localization.en
dc.description.sponsorshipBBSRC, UK; National Heart, Lung and Blood Institute and Drexel University College of Medicine Clinical & Translational Research Institute, CTRIUSA Award Number R01HL107771; EPSRC E-TERM Landscape Fellowship (EP/I017801/1)en
dc.language.isoenen
dc.publisherFuture Medicineen
dc.relation.urlhttp://www.futuremedicine.com/doi/10.2217/nnm.15.202en
dc.subjectastrocytesen
dc.subjectcell transplantationen
dc.subjectlabel dilutionen
dc.subjectmagnetiteen
dc.subjectmagnetolabelingen
dc.subjectpolymeric particlesen
dc.titleEndocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritanceen
dc.typeArticleen
dc.identifier.eissn1748-6963en
dc.contributor.departmentKeele University, United Kingdom; Drexel University College of Medicine, Philadelphia, USAen
dc.identifier.journalNanomedicineen

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