Interaction of a dengue virus NS1-derived peptide with the inhibitory receptor KIR3DL1 on natural killer cells

Hdl Handle:
http://hdl.handle.net/10034/604234
Title:
Interaction of a dengue virus NS1-derived peptide with the inhibitory receptor KIR3DL1 on natural killer cells
Authors:
Townsley, Elizabeth; O'Connor, Geraldine M.; Cosgrove, Cormac; Woda, Marcia; Co, Mary; Thomas, Stephen J.; Kalayanarooj, Siripen; Yoon, In-Kyu; Nisalak, Ananda; Srikiatkhachorn, Anon; Green, Sharone; Stephens, Henry A. F.; Gostick, Emma; Price, David A.; Carrington, Mary; Alter, Galit; McVicar, Daniel W.; Rothman, Alan L.; Mathew, Anuja
Abstract:
Killer immunoglobulin-like receptors (KIRs) interact with human leucocyte antigen (HLA) class I ligands and play a key role in the regulation and activation of NK cells. The functional importance of KIR-HLA interactions has been demonstrated for a number of chronic viral infections, but to date only a few studies have been performed in the context of acute self-limited viral infections. During our investigation of CD8(+) T cell responses to a conserved HLA-B57-restricted epitope derived from dengue virus (DENV) non-structural protein-1 (NS1), we observed substantial binding of the tetrameric complex to non-T/non-B lymphocytes in peripheral blood mononuclear cells (PBMC) from a long-standing clinical cohort in Thailand. We confirmed binding of the NS1 tetramer to CD56(dim) NK cells, which are known to express KIRs. Using depletion studies and KIR-transfected cell lines, we demonstrated further that the NS1 tetramer bound the inhibitory receptor KIR3DL1. Phenotypical analysis of PBMC from HLA-B57(+) subjects with acute DENV infection revealed marked activation of NS1 tetramer-binding natural killer (NK) cells around the time of defervescence in subjects with severe dengue disease. Collectively, our findings indicate that subsets of NK cells are activated relatively late in the course of acute DENV illness and reveal a possible role for specific KIR-HLA interactions in the modulation of disease outcomes.
Affiliation:
Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA, USA. Cancer and Inflammation Program, Laboratory of Experimental Immunology, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, USA. Ragon Institute at MGH, MIT And Harvard, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. Walter Reed Army Institute of Research, Silver Spring, MD, USA. Queen Sirikit National Institute for Child Health, Bangkok, Thailand. Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand. Centre for Nephrology and the Anthony Nolan Trust, Royal Free Campus, University College, London, UK. Cardiff University School of Medicine, Institute of Infection and Immunity, Cardiff, UK. Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Citation:
Townsley, E., O'Connor, G., Cosgrove, C., Woda, M., Co, M., Thomas, S. J., . . . Mathew, A. (2016). Interaction of a dengue virus NS1-derived peptide with the inhibitory receptor KIR3DL1 on natural killer cells. Clinical & Experimental Immunology, 183(3), 419-430. doi: 10.1111/cei.12722
Journal:
Clinical and Experimental Immunology
Publication Date:
Nov-2015
URI:
http://hdl.handle.net/10034/604234
DOI:
10.1111/cei.12722
Type:
Article
Language:
en_US
Appears in Collections:
Biological Sciences

Full metadata record

DC FieldValue Language
dc.contributor.authorTownsley, Elizabethen
dc.contributor.authorO'Connor, Geraldine M.en
dc.contributor.authorCosgrove, Cormacen
dc.contributor.authorWoda, Marciaen
dc.contributor.authorCo, Maryen
dc.contributor.authorThomas, Stephen J.en
dc.contributor.authorKalayanarooj, Siripenen
dc.contributor.authorYoon, In-Kyuen
dc.contributor.authorNisalak, Anandaen
dc.contributor.authorSrikiatkhachorn, Anonen
dc.contributor.authorGreen, Sharoneen
dc.contributor.authorStephens, Henry A. F.en
dc.contributor.authorGostick, Emmaen
dc.contributor.authorPrice, David A.en
dc.contributor.authorCarrington, Maryen
dc.contributor.authorAlter, Galiten
dc.contributor.authorMcVicar, Daniel W.en
dc.contributor.authorRothman, Alan L.en
dc.contributor.authorMathew, Anujaen
dc.date.accessioned2016-04-01T14:54:36Zen
dc.date.available2016-04-01T14:54:36Zen
dc.date.issued2015-11en
dc.identifier.citationTownsley, E., O'Connor, G., Cosgrove, C., Woda, M., Co, M., Thomas, S. J., . . . Mathew, A. (2016). Interaction of a dengue virus NS1-derived peptide with the inhibitory receptor KIR3DL1 on natural killer cells. Clinical & Experimental Immunology, 183(3), 419-430. doi: 10.1111/cei.12722en
dc.identifier.doi10.1111/cei.12722en
dc.identifier.urihttp://hdl.handle.net/10034/604234en
dc.description.abstractKiller immunoglobulin-like receptors (KIRs) interact with human leucocyte antigen (HLA) class I ligands and play a key role in the regulation and activation of NK cells. The functional importance of KIR-HLA interactions has been demonstrated for a number of chronic viral infections, but to date only a few studies have been performed in the context of acute self-limited viral infections. During our investigation of CD8(+) T cell responses to a conserved HLA-B57-restricted epitope derived from dengue virus (DENV) non-structural protein-1 (NS1), we observed substantial binding of the tetrameric complex to non-T/non-B lymphocytes in peripheral blood mononuclear cells (PBMC) from a long-standing clinical cohort in Thailand. We confirmed binding of the NS1 tetramer to CD56(dim) NK cells, which are known to express KIRs. Using depletion studies and KIR-transfected cell lines, we demonstrated further that the NS1 tetramer bound the inhibitory receptor KIR3DL1. Phenotypical analysis of PBMC from HLA-B57(+) subjects with acute DENV infection revealed marked activation of NS1 tetramer-binding natural killer (NK) cells around the time of defervescence in subjects with severe dengue disease. Collectively, our findings indicate that subsets of NK cells are activated relatively late in the course of acute DENV illness and reveal a possible role for specific KIR-HLA interactions in the modulation of disease outcomes.en
dc.language.isoen_USen
dc.subjectNatural Killer Cellsen
dc.subjectKIRen
dc.subjectDengue feveren
dc.titleInteraction of a dengue virus NS1-derived peptide with the inhibitory receptor KIR3DL1 on natural killer cellsen_US
dc.typeArticleen
dc.contributor.departmentDivision of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA, USA. Cancer and Inflammation Program, Laboratory of Experimental Immunology, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, USA. Ragon Institute at MGH, MIT And Harvard, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. Walter Reed Army Institute of Research, Silver Spring, MD, USA. Queen Sirikit National Institute for Child Health, Bangkok, Thailand. Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand. Centre for Nephrology and the Anthony Nolan Trust, Royal Free Campus, University College, London, UK. Cardiff University School of Medicine, Institute of Infection and Immunity, Cardiff, UK. Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.en
dc.identifier.journalClinical and Experimental Immunologyen
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