Total hip and knee replacement surgery results in changes in leukocyte and endothelial markers

Hdl Handle:
http://hdl.handle.net/10034/604069
Title:
Total hip and knee replacement surgery results in changes in leukocyte and endothelial markers
Authors:
Hughes, Stephen F.; Hendricks, Beverly D.; Edwards, David R.; Maclean, Kirsty M.; Bastawrous, Salah S.; Middleton, Jim F.
Abstract:
BACKGROUND: It is estimated that over 8 million people in the United Kingdom suffer from osteoarthritis. These patients may require orthopaedic surgical intervention to help alleviate their clinical condition. Investigations presented here was to test the hypothesis that total hip replacement (THR) and total knee replacement (TKR) orthopaedic surgery result in changes to leukocyte and endothelial markers thus increasing inflammatory reactions postoperatively. METHODS: During this 'pilot study', ten test subjects were all scheduled for THR or TKR elective surgery due to osteoarthritis. Leukocyte concentrations were measured using an automated full blood count analyser. Leukocyte CD11b (Mac-1) and CD62L cell surface expression, intracellular production of H(2)O(2 )and elastase were measured as markers of leukocyte function. Von Willebrand factor (vWF) and soluble intercellular adhesion molecule-1 (sICAM-1) were measured as markers of endothelial activation. RESULTS: The results obtained during this study demonstrate that THR and TKR orthopaedic surgery result in similar changes of leukocyte and endothelial markers, suggestive of increased inflammatory reactions postoperatively. Specifically, THR and TKR surgery resulted in a leukocytosis, this being demonstrated by an increase in the total leukocyte concentration following surgery. Evidence of leukocyte activation was demonstrated by a decrease in CD62L expression and an increase in CD11b expression by neutrophils and monocytes respectively. An increase in the intracellular H(2)O(2 )production by neutrophils and monocytes and in the leukocyte elastase concentrations was also evident of leukocyte activation following orthopaedic surgery. With respect to endothelial activation, increases in vWF and sICAM-1 concentrations were demonstrated following surgery. CONCLUSION: In general it appeared that most of the leukocyte and endothelial markers measured during these studies peaked between days 1-3 postoperatively. It is proposed that by allowing orthopaedic surgeons access to alternative laboratory markers such as CD11b, H(2)O(2 )and elastase, CD62L, vWF and sICAM-1, an accurate assessment of the extent of inflammation due to surgery per se could be made. Ultimately, the leukocyte and endothelial markers assessed during this investigation may have a role in monitoring potential infectious complications that can occur during the postoperative period.
Affiliation:
University of Chester; North Wales (Central) NHS Trust; Keele University
Citation:
Hughes, S. F., Beverly, D., Hendricks, B. D., David, R., Edwards, D. R., Kirsty, M., & Middleton, J. F. S. (2010). Total hip and knee replacement surgery results in changes in leukocyte and endothelial markers. Journal of Inflammation, 7, 1-12. DOI: 10.1186/1476-9255-7-2
Publisher:
BioMed Central
Journal:
Journal of Inflammation
Publication Date:
19-Jan-2010
URI:
http://hdl.handle.net/10034/604069
DOI:
10.1186/1476-9255-7-2
Additional Links:
http://www.ncbi.nlm.nih.gov/pubmed/20148137; http://journal-inflammation.biomedcentral.com/articles/10.1186/1476-9255-7-2
Type:
Article
Language:
en
Description:
Original Research Article
EISSN:
1476-9255
Sponsors:
Institute of Biomedical Science (IBMS) and the North Wales Research Committee (NWRC) for their financial support.
Appears in Collections:
Biological Sciences

Full metadata record

DC FieldValue Language
dc.contributor.authorHughes, Stephen F.en
dc.contributor.authorHendricks, Beverly D.en
dc.contributor.authorEdwards, David R.en
dc.contributor.authorMaclean, Kirsty M.en
dc.contributor.authorBastawrous, Salah S.en
dc.contributor.authorMiddleton, Jim F.en
dc.date.accessioned2016-03-31T14:56:27Zen
dc.date.available2016-03-31T14:56:27Zen
dc.date.issued2010-01-19en
dc.identifier.citationHughes, S. F., Beverly, D., Hendricks, B. D., David, R., Edwards, D. R., Kirsty, M., & Middleton, J. F. S. (2010). Total hip and knee replacement surgery results in changes in leukocyte and endothelial markers. Journal of Inflammation, 7, 1-12. DOI: 10.1186/1476-9255-7-2en
dc.identifier.doi10.1186/1476-9255-7-2en
dc.identifier.urihttp://hdl.handle.net/10034/604069en
dc.descriptionOriginal Research Articleen
dc.description.abstractBACKGROUND: It is estimated that over 8 million people in the United Kingdom suffer from osteoarthritis. These patients may require orthopaedic surgical intervention to help alleviate their clinical condition. Investigations presented here was to test the hypothesis that total hip replacement (THR) and total knee replacement (TKR) orthopaedic surgery result in changes to leukocyte and endothelial markers thus increasing inflammatory reactions postoperatively. METHODS: During this 'pilot study', ten test subjects were all scheduled for THR or TKR elective surgery due to osteoarthritis. Leukocyte concentrations were measured using an automated full blood count analyser. Leukocyte CD11b (Mac-1) and CD62L cell surface expression, intracellular production of H(2)O(2 )and elastase were measured as markers of leukocyte function. Von Willebrand factor (vWF) and soluble intercellular adhesion molecule-1 (sICAM-1) were measured as markers of endothelial activation. RESULTS: The results obtained during this study demonstrate that THR and TKR orthopaedic surgery result in similar changes of leukocyte and endothelial markers, suggestive of increased inflammatory reactions postoperatively. Specifically, THR and TKR surgery resulted in a leukocytosis, this being demonstrated by an increase in the total leukocyte concentration following surgery. Evidence of leukocyte activation was demonstrated by a decrease in CD62L expression and an increase in CD11b expression by neutrophils and monocytes respectively. An increase in the intracellular H(2)O(2 )production by neutrophils and monocytes and in the leukocyte elastase concentrations was also evident of leukocyte activation following orthopaedic surgery. With respect to endothelial activation, increases in vWF and sICAM-1 concentrations were demonstrated following surgery. CONCLUSION: In general it appeared that most of the leukocyte and endothelial markers measured during these studies peaked between days 1-3 postoperatively. It is proposed that by allowing orthopaedic surgeons access to alternative laboratory markers such as CD11b, H(2)O(2 )and elastase, CD62L, vWF and sICAM-1, an accurate assessment of the extent of inflammation due to surgery per se could be made. Ultimately, the leukocyte and endothelial markers assessed during this investigation may have a role in monitoring potential infectious complications that can occur during the postoperative period.en
dc.description.sponsorshipInstitute of Biomedical Science (IBMS) and the North Wales Research Committee (NWRC) for their financial support.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/20148137en
dc.relation.urlhttp://journal-inflammation.biomedcentral.com/articles/10.1186/1476-9255-7-2en
dc.subjectOrthopaedic surgeryen
dc.subjectInflammationen
dc.titleTotal hip and knee replacement surgery results in changes in leukocyte and endothelial markersen
dc.typeArticleen
dc.identifier.eissn1476-9255en
dc.contributor.departmentUniversity of Chester; North Wales (Central) NHS Trust; Keele Universityen
dc.identifier.journalJournal of Inflammationen
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