Spatial variation in species composition of Saprolegnia, a parasitic oomycete of amphibian eggs, in Scotland

Hdl Handle:
http://hdl.handle.net/10034/600704
Title:
Spatial variation in species composition of Saprolegnia, a parasitic oomycete of amphibian eggs, in Scotland
Authors:
Muir, Anna P. ( 0000-0002-6896-6915 ) ; Kilbride, Elizabeth; Mable, Barbara K.
Abstract:
Parasitic water moulds in the genus Saprolegnia cause mortality of amphibian embryos and reduced size at metamorphosis, leading to increased adult mortality. Most studies of virulence have focused on only a single Saprolegnia species, but the Saprolegnia species associated with amphibian eggs and their distributions are not well known. This study aimed to investigate the distribution of amphibian-associated water moulds in Scotland. In particular, we asked the questions: i) Does Saprolegnia species composition vary between sites?; and ii) Is presence of Saprolegnia related to environmental parameters? Common frog (Rana temporaria) eggs with evidence of Saprolegnia infection were sampled from ten sites, cultured, and the 28S region of the rDNA array sequenced. Thirteen samples isolated from four sites were identified as members of the Saprolegniaceae and the ITS region of these samples were subsequently sequenced to further resolve species identification. Four species of Saprolegnia were found in total, with one or two species of Saprolegnia present in each of four sites. S. diclina was the most common species identified and was found at three of the four sites. Acidity was significantly lower and altitude significantly higher at sites where Saprolegniaceae were present. Therefore, R. temporaria eggs in different pools are subject to infection by different, and in some instances more than one, species of Saprolegnia. Overall, our findings suggest that future studies of virulence need to consider the effect of multiple Saprolegnia species within a site as well as the population of origin of the amphibian host
Affiliation:
University of Chester; University of Glasgow
Citation:
Muir, A. P., Kilbride, E., & Mable, B. K. (2015). Spatial variation in species composition of Saprolegnia, a parasitic oomycete of amphibian eggs, in Scotland. Herpetological Journal, 25(4), 257–263.
Publisher:
The British Herpetological Society
Journal:
Herpetological Journal
Publication Date:
1-Oct-2015
URI:
http://hdl.handle.net/10034/600704
Additional Links:
http://www.ingentaconnect.com/content/bhs/thj/2015/00000025/00000004/art00007; http://www.thebhs.org/index.php?option=com_docman&Itemid=42
Type:
Article
Language:
en
Description:
This document is the Accepted Manuscript version of a published work that appeared in final form in Herpetological Journal. To access the final edited and published work see http://www.thebhs.org/index.php?option=com_docman&Itemid=42.
ISSN:
0268-0130
Sponsors:
Fieldwork was supported by grants from the Royal Geographic Society, the Glasgow Natural History Society and the Scottish Mountaineering Trust. This study was supported by PhD CASE studentship funding from the Biotechnology and Biological Sciences Research Council, in partnership with the Royal Zoological Society of Scotland.
Appears in Collections:
Biological Sciences

Full metadata record

DC FieldValue Language
dc.contributor.authorMuir, Anna P.en
dc.contributor.authorKilbride, Elizabethen
dc.contributor.authorMable, Barbara K.en
dc.date.accessioned2016-03-07T12:48:27Zen
dc.date.available2016-03-07T12:48:27Zen
dc.date.issued2015-10-01en
dc.identifier.citationMuir, A. P., Kilbride, E., & Mable, B. K. (2015). Spatial variation in species composition of Saprolegnia, a parasitic oomycete of amphibian eggs, in Scotland. Herpetological Journal, 25(4), 257–263.en
dc.identifier.issn0268-0130en
dc.identifier.urihttp://hdl.handle.net/10034/600704en
dc.descriptionThis document is the Accepted Manuscript version of a published work that appeared in final form in Herpetological Journal. To access the final edited and published work see http://www.thebhs.org/index.php?option=com_docman&Itemid=42.-
dc.description.abstractParasitic water moulds in the genus Saprolegnia cause mortality of amphibian embryos and reduced size at metamorphosis, leading to increased adult mortality. Most studies of virulence have focused on only a single Saprolegnia species, but the Saprolegnia species associated with amphibian eggs and their distributions are not well known. This study aimed to investigate the distribution of amphibian-associated water moulds in Scotland. In particular, we asked the questions: i) Does Saprolegnia species composition vary between sites?; and ii) Is presence of Saprolegnia related to environmental parameters? Common frog (Rana temporaria) eggs with evidence of Saprolegnia infection were sampled from ten sites, cultured, and the 28S region of the rDNA array sequenced. Thirteen samples isolated from four sites were identified as members of the Saprolegniaceae and the ITS region of these samples were subsequently sequenced to further resolve species identification. Four species of Saprolegnia were found in total, with one or two species of Saprolegnia present in each of four sites. S. diclina was the most common species identified and was found at three of the four sites. Acidity was significantly lower and altitude significantly higher at sites where Saprolegniaceae were present. Therefore, R. temporaria eggs in different pools are subject to infection by different, and in some instances more than one, species of Saprolegnia. Overall, our findings suggest that future studies of virulence need to consider the effect of multiple Saprolegnia species within a site as well as the population of origin of the amphibian hosten
dc.description.sponsorshipFieldwork was supported by grants from the Royal Geographic Society, the Glasgow Natural History Society and the Scottish Mountaineering Trust. This study was supported by PhD CASE studentship funding from the Biotechnology and Biological Sciences Research Council, in partnership with the Royal Zoological Society of Scotland.en
dc.language.isoenen
dc.publisherThe British Herpetological Societyen
dc.relation.urlhttp://www.ingentaconnect.com/content/bhs/thj/2015/00000025/00000004/art00007en
dc.relation.urlhttp://www.thebhs.org/index.php?option=com_docman&Itemid=42en
dc.subjectevolutionary biologyen
dc.subjectpopulation geneticsen
dc.subjectconservation biologyen
dc.subjectdisease ecologyen
dc.titleSpatial variation in species composition of Saprolegnia, a parasitic oomycete of amphibian eggs, in Scotlanden
dc.typeArticleen
dc.contributor.departmentUniversity of Chester; University of Glasgowen
dc.identifier.journalHerpetological Journalen
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