Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response

Hdl Handle:
http://hdl.handle.net/10034/577147
Title:
Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response
Authors:
Waugh, David G.; Lawrence, Jonathan; Shukla, Pratik; Chan, Chi-Wai; Hussain, Issam; Man, Hau-Chung; Smith, Graham C.
Abstract:
Mesenchymal stem cells (MSCs) are known to play important roles in development, post-natal growth, repair, and regeneration of mesenchymal tissues. What is more, surface treatments are widely reported to affect the biomimetic nature of materials. This paper will detail, discuss and compare laser surface treatment of polyamide (Polyamide 6,6), using a 60 W CO2 laser, and NiTi alloy, using a 100 W fiber laser, and the effects of these treatments on mesenchymal stem cell response. The surface morphology and composition of the polyamide and NiTi alloy were studied by scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS), respectively. MSC cell morphology cell counting and viability measurements were done by employing a haemocytometer and MTT colorimetric assay. The success of enhanced adhesion and spreading of the MSCs on each of the laser surface treated samples, when compared to as-received samples, is evidenced in this work.
Affiliation:
University of Chester (Waugh, Lawrence, Shukla, Smith), Queen's University Belfast (Chan), University of Lincoln (Hussain), Hong Kong Polytechnica University (Man)
Citation:
Waugh, D. G., Lawrence, J., Shukla, P., Chan, C., Hussain, I., Man, H. C., & Smith, G. C. (2015). Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response. Proceedings of the SPIE, 9657. Green, M. & Rose, C. (Eds). 86-92 (2015)
Publisher:
SPIE
Publication Date:
2015
URI:
http://hdl.handle.net/10034/577147
Additional Links:
http://spie.org/x4325.xml?start_volume_number=9600&end_volume_number=9668
Type:
Article
Language:
en
Description:
Copyright 2015 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/12.2182432
Appears in Collections:
Natural Sciences

Full metadata record

DC FieldValue Language
dc.contributor.authorWaugh, David G.en
dc.contributor.authorLawrence, Jonathanen
dc.contributor.authorShukla, Pratiken
dc.contributor.authorChan, Chi-Waien
dc.contributor.authorHussain, Issamen
dc.contributor.authorMan, Hau-Chungen
dc.contributor.authorSmith, Graham C.en
dc.date.accessioned2015-09-10T15:03:18Zen
dc.date.available2015-09-10T15:03:18Zen
dc.date.issued2015en
dc.identifier.citationWaugh, D. G., Lawrence, J., Shukla, P., Chan, C., Hussain, I., Man, H. C., & Smith, G. C. (2015). Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response. Proceedings of the SPIE, 9657. Green, M. & Rose, C. (Eds). 86-92 (2015)en
dc.identifier.urihttp://hdl.handle.net/10034/577147en
dc.descriptionCopyright 2015 Society of Photo Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/12.2182432en
dc.description.abstractMesenchymal stem cells (MSCs) are known to play important roles in development, post-natal growth, repair, and regeneration of mesenchymal tissues. What is more, surface treatments are widely reported to affect the biomimetic nature of materials. This paper will detail, discuss and compare laser surface treatment of polyamide (Polyamide 6,6), using a 60 W CO2 laser, and NiTi alloy, using a 100 W fiber laser, and the effects of these treatments on mesenchymal stem cell response. The surface morphology and composition of the polyamide and NiTi alloy were studied by scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS), respectively. MSC cell morphology cell counting and viability measurements were done by employing a haemocytometer and MTT colorimetric assay. The success of enhanced adhesion and spreading of the MSCs on each of the laser surface treated samples, when compared to as-received samples, is evidenced in this work.en
dc.language.isoenen
dc.publisherSPIEen
dc.relation.urlhttp://spie.org/x4325.xml?start_volume_number=9600&end_volume_number=9668en
dc.titleLaser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell responseen
dc.typeArticleen
dc.contributor.departmentUniversity of Chester (Waugh, Lawrence, Shukla, Smith), Queen's University Belfast (Chan), University of Lincoln (Hussain), Hong Kong Polytechnica University (Man)en
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