Model-directed engineering of “difficult-to-express” monoclonal antibody production by Chinese hamster ovary cells

Hdl Handle:
http://hdl.handle.net/10034/552294
Title:
Model-directed engineering of “difficult-to-express” monoclonal antibody production by Chinese hamster ovary cells
Authors:
Pybus, Leon P.; Dean, Greg; West, Nathan R.; Smith, Andrew; Daramola, Olalekan; Field, Ray; Wilkinson, Stephen J.; James, David C.
Abstract:
Despite improvements in volumetric titer for monoclonal antibody (MAb) production processes using Chinese hamster ovary (CHO) cells, some “difficult-to-express” (DTE) MAbs inexplicably reach much lower process titers.
Affiliation:
University of Sheffield ; BioPharmaceutical Development ; University of Sheffield ; BioPharmaceutical Development ; BioPharmaceutical Development ; BioPharmaceutical Development ; University of Sheffield ; University of Sheffield
Citation:
Model-directed engineering of “difficult-to-express” monoclonal antibody production by Chinese hamster ovary cells. Biotechnology and Bioengineering, 2014, 111(2), pp. 372-385
Publisher:
Wiley
Journal:
Biotechnology and Bioengineering
Publication Date:
14-Nov-2013
URI:
http://hdl.handle.net/10034/552294
DOI:
10.1002/bit.25116
Additional Links:
http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0290; http://doi.wiley.com/10.1002/bit.25116
Type:
Article
Language:
en
Description:
This article is not available through ChesterRep.
ISSN:
0006-3592
Appears in Collections:
Chemical Engineering

Full metadata record

DC FieldValue Language
dc.contributor.authorPybus, Leon P.en
dc.contributor.authorDean, Gregen
dc.contributor.authorWest, Nathan R.en
dc.contributor.authorSmith, Andrewen
dc.contributor.authorDaramola, Olalekanen
dc.contributor.authorField, Rayen
dc.contributor.authorWilkinson, Stephen J.en
dc.contributor.authorJames, David C.en
dc.date.accessioned2015-05-05T13:02:43Zen
dc.date.available2015-05-05T13:02:43Zen
dc.date.issued2013-11-14en
dc.identifier.citationModel-directed engineering of “difficult-to-express” monoclonal antibody production by Chinese hamster ovary cells. Biotechnology and Bioengineering, 2014, 111(2), pp. 372-385en
dc.identifier.issn0006-3592en
dc.identifier.doi10.1002/bit.25116en
dc.identifier.urihttp://hdl.handle.net/10034/552294en
dc.descriptionThis article is not available through ChesterRep.en
dc.description.abstractDespite improvements in volumetric titer for monoclonal antibody (MAb) production processes using Chinese hamster ovary (CHO) cells, some “difficult-to-express” (DTE) MAbs inexplicably reach much lower process titers.en
dc.language.isoenen
dc.publisherWileyen
dc.relation.urlhttp://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0290en
dc.relation.urlhttp://doi.wiley.com/10.1002/bit.25116en
dc.rightsArchived with thanks to Biotechnology and Bioengineeringen
dc.subjectChinese hamster ovary cellsen
dc.subjectrecombinant monoclonal antibodyen
dc.subjectmathematical modelingen
dc.subjectunfolded protein responseen
dc.subjectdifficult-to-express proteinsen
dc.subjectcell line engineeringen
dc.titleModel-directed engineering of “difficult-to-express” monoclonal antibody production by Chinese hamster ovary cellsen
dc.typeArticleen
dc.contributor.departmentUniversity of Sheffield ; BioPharmaceutical Development ; University of Sheffield ; BioPharmaceutical Development ; BioPharmaceutical Development ; BioPharmaceutical Development ; University of Sheffield ; University of Sheffielden
dc.identifier.journalBiotechnology and Bioengineeringen
dc.contributor.institutionChELSI Institute; Department of Chemical and Biological Engineering; University of Sheffield; Mappin Street Sheffield S1 3JD UKen
dc.contributor.institutionCell Sciences; BioPharmaceutical Development, MedImmune, Granta Park; Cambridge UKen
dc.contributor.institutionChELSI Institute; Department of Chemical and Biological Engineering; University of Sheffield; Mappin Street Sheffield S1 3JD UKen
dc.contributor.institutionCell Sciences; BioPharmaceutical Development, MedImmune, Granta Park; Cambridge UKen
dc.contributor.institutionCell Sciences; BioPharmaceutical Development, MedImmune, Granta Park; Cambridge UKen
dc.contributor.institutionCell Sciences; BioPharmaceutical Development, MedImmune, Granta Park; Cambridge UKen
dc.contributor.institutionChELSI Institute; Department of Chemical and Biological Engineering; University of Sheffield; Mappin Street Sheffield S1 3JD UKen
dc.contributor.institutionChELSI Institute; Department of Chemical and Biological Engineering; University of Sheffield; Mappin Street Sheffield S1 3JD UKen
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