Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response

Hdl Handle:
http://hdl.handle.net/10034/550452
Title:
Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response
Authors:
Waugh, David G.; Lawrence, Jonathan; Shukla, Pratik; Chan, Chi-Wai; Hussain, Issam; Man, Hau-Chung; Smith, Graham C.
Abstract:
Mesenchymal stem cells (MSCs) are known to play important roles in development, post-natal growth, repair, and regeneration of mesenchymal tissues. What is more, surface treatments are widely reported to affect the biomimetic nature of materials. This paper will detail, discuss and compare laser surface treatment of polyamide (Polyamide 6,6), using a 60 W CO2 laser, and NiTi alloy, using a 100 W fiber laser, and the effects of these treatments on mesenchymal stem cell response. The surface morphology and composition of the polyamide and NiTi alloy were studied by scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS), respectively. MSC cell morphology cell counting and viability measurements were done by employing a haemocytometer and MTT colorimetric assay. The success of enhanced adhesion and spreading of the MSCs on each of the laser surface treated samples, when compared to as-received samples, is evidenced in this work.
Affiliation:
University of Chester ; University of Chester ; University of Chester ; Queen's University, Belfast ; University of Lincoln ; Hong Kong Polytechnic University ; University of Chester
Citation:
Waugh, D. G., Lawrence, J., Shukla P., Chan, C. W., Hussain, I., Man, H. C., & Smith, G. C. (2015). Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response. Unpublished conference paper given at the Industrial Laser Applications Symposium at Chesford Grange Conference Centre, Kenilworth, UK, 17-18 March 2015.
Publication Date:
18-Mar-2015
URI:
http://hdl.handle.net/10034/550452
Additional Links:
http://ilas2015.co.uk
Type:
Presentation; Meetings and Proceedings
Language:
en
Appears in Collections:
Mechanical Engineering

Full metadata record

DC FieldValue Language
dc.contributor.authorWaugh, David G.en
dc.contributor.authorLawrence, Jonathanen
dc.contributor.authorShukla, Pratiken
dc.contributor.authorChan, Chi-Waien
dc.contributor.authorHussain, Issamen
dc.contributor.authorMan, Hau-Chungen
dc.contributor.authorSmith, Graham C.en
dc.date.accessioned2015-04-22T14:27:49Zen
dc.date.available2015-04-22T14:27:49Zen
dc.date.issued2015-03-18en
dc.identifier.citationWaugh, D. G., Lawrence, J., Shukla P., Chan, C. W., Hussain, I., Man, H. C., & Smith, G. C. (2015). Laser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell response. Unpublished conference paper given at the Industrial Laser Applications Symposium at Chesford Grange Conference Centre, Kenilworth, UK, 17-18 March 2015.en
dc.identifier.urihttp://hdl.handle.net/10034/550452en
dc.description.abstractMesenchymal stem cells (MSCs) are known to play important roles in development, post-natal growth, repair, and regeneration of mesenchymal tissues. What is more, surface treatments are widely reported to affect the biomimetic nature of materials. This paper will detail, discuss and compare laser surface treatment of polyamide (Polyamide 6,6), using a 60 W CO2 laser, and NiTi alloy, using a 100 W fiber laser, and the effects of these treatments on mesenchymal stem cell response. The surface morphology and composition of the polyamide and NiTi alloy were studied by scanning electron microscopy (SEM) and X-ray photoemission spectroscopy (XPS), respectively. MSC cell morphology cell counting and viability measurements were done by employing a haemocytometer and MTT colorimetric assay. The success of enhanced adhesion and spreading of the MSCs on each of the laser surface treated samples, when compared to as-received samples, is evidenced in this work.en
dc.language.isoenen
dc.relation.urlhttp://ilas2015.co.uken
dc.subjectstem cellsen
dc.subjectcell viabilityen
dc.subjectlaser surface treatmenten
dc.subjectCO2 laseren
dc.subjectfiber laseren
dc.subjectMTTen
dc.titleLaser surface treatment of polyamide and NiTi alloy and the effects on mesenchymal stem cell responseen
dc.typePresentationen
dc.typeMeetings and Proceedingsen
dc.contributor.departmentUniversity of Chester ; University of Chester ; University of Chester ; Queen's University, Belfast ; University of Lincoln ; Hong Kong Polytechnic University ; University of Chesteren
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