Monocytes/macrophages express CCR9 in rheumatoid arthritis and CCL25 stimulates their differentiation

Hdl Handle:
http://hdl.handle.net/10034/279274
Title:
Monocytes/macrophages express CCR9 in rheumatoid arthritis and CCL25 stimulates their differentiation
Authors:
Schmutz, Caroline; Cartwright, Alison; Williams, Helen; Haworth, Oliver; Williams, John H. H.; Filer, Andrew; Salmon, Mike; Buckley, Christopher D.; Middleton, Jim F.
Abstract:
Abstract Introduction Monocytes/macrophages accumulate in the rheumatoid (RA) synovium where they play a central role in inflammation and joint destruction. Identification of molecules involved in their accumulation and differentiation is important to inform therapeutic strategies. This study investigated the expression and function of chemokine receptor CCR9 in the peripheral blood (PB) and synovium of RA, non-RA patients and healthy volunteers. Methods CCR9 expression on PB monocytes/macrophages was analysed by flow cytometry and in synovium by immunofluorescence. Chemokine receptor CCR9 mRNA expression was examined in RA and non-RA synovium, monocytes/macrophages from PB and synovial fluid (SF) of RA patients and PB of healthy donors using the reverse transcription polymerase chain reaction (RT-PCR). Monocyte differentiation and chemotaxis to chemokine ligand 25 (CCL25)/TECK were used to study CCR9 function. Results CCR9 was expressed by PB monocytes/macrophages in RA and healthy donors, and increased in RA. In RA and non-RA synovia, CCR9 co-localised with cluster of differentiation 14+ (CD14+) and cluster of differentiation 68+ (CD68+) macrophages, and was more abundant in RA synovium. CCR9 mRNA was detected in the synovia of all RA patients and in some non-RA controls, and monocytes/macrophages from PB and SF of RA and healthy controls. CCL25 was detected in RA and non-RA synovia where it co-localised with CD14+ and CD68+ cells. Tumour necrosis factor alpha (TNFα) increased CCR9 expression on human acute monocytic leukemia cell line THP-1 monocytic cells. CCL25 induced a stronger monocyte differentiation in RA compared to healthy donors. CCL25 induced significant chemotaxis of PB monocytes but not consistently among individuals. Conclusions CCR9 expression by monocytes is increased in RA. CCL25 may be involved in the differentiation of monocytes to macrophages particularly in RA.
Affiliation:
Keele University/University of Birmingham ; Keele University ; University of Chester ; University of Birmingham ; University of Chester ; University of Birmingham ; University of Birmingham ; University of Birmingham ; Keele University/University of Bristol
Citation:
Arthritis Research & Therapy, 2010, 12(4), R161
Publisher:
BioMed Central
Journal:
Arthritis Research & Therapy
Publication Date:
25-Aug-2010
DOI:
10.1186/ar3120
Additional Links:
http://arthritis-research.com
Type:
Article
Language:
en
Description:
Available Gold OA
ISSN:
1478-6354
Appears in Collections:
Biological Sciences; Biological Sciences; Gold OA

Full metadata record

DC FieldValue Language
dc.contributor.authorSchmutz, Carolineen_GB
dc.contributor.authorCartwright, Alisonen_GB
dc.contributor.authorWilliams, Helenen_GB
dc.contributor.authorHaworth, Oliveren_GB
dc.contributor.authorWilliams, John H. H.en_GB
dc.contributor.authorFiler, Andrewen_GB
dc.contributor.authorSalmon, Mikeen_GB
dc.contributor.authorBuckley, Christopher D.en_GB
dc.contributor.authorMiddleton, Jim F.en_GB
dc.date.accessioned2013-04-08T09:20:15Zen
dc.date.available2013-04-08T09:20:15Zen
dc.date.issued2010-08-25en
dc.identifier.citationArthritis Research & Therapy, 2010, 12(4), R161en_GB
dc.identifier.issn1478-6354en
dc.identifier.doi10.1186/ar3120-
dc.descriptionAvailable Gold OAen_GB
dc.description.abstractAbstract Introduction Monocytes/macrophages accumulate in the rheumatoid (RA) synovium where they play a central role in inflammation and joint destruction. Identification of molecules involved in their accumulation and differentiation is important to inform therapeutic strategies. This study investigated the expression and function of chemokine receptor CCR9 in the peripheral blood (PB) and synovium of RA, non-RA patients and healthy volunteers. Methods CCR9 expression on PB monocytes/macrophages was analysed by flow cytometry and in synovium by immunofluorescence. Chemokine receptor CCR9 mRNA expression was examined in RA and non-RA synovium, monocytes/macrophages from PB and synovial fluid (SF) of RA patients and PB of healthy donors using the reverse transcription polymerase chain reaction (RT-PCR). Monocyte differentiation and chemotaxis to chemokine ligand 25 (CCL25)/TECK were used to study CCR9 function. Results CCR9 was expressed by PB monocytes/macrophages in RA and healthy donors, and increased in RA. In RA and non-RA synovia, CCR9 co-localised with cluster of differentiation 14+ (CD14+) and cluster of differentiation 68+ (CD68+) macrophages, and was more abundant in RA synovium. CCR9 mRNA was detected in the synovia of all RA patients and in some non-RA controls, and monocytes/macrophages from PB and SF of RA and healthy controls. CCL25 was detected in RA and non-RA synovia where it co-localised with CD14+ and CD68+ cells. Tumour necrosis factor alpha (TNFα) increased CCR9 expression on human acute monocytic leukemia cell line THP-1 monocytic cells. CCL25 induced a stronger monocyte differentiation in RA compared to healthy donors. CCL25 induced significant chemotaxis of PB monocytes but not consistently among individuals. Conclusions CCR9 expression by monocytes is increased in RA. CCL25 may be involved in the differentiation of monocytes to macrophages particularly in RA.en_GB
dc.language.isoenen
dc.publisherBioMed Centralen_GB
dc.relation.urlhttp://arthritis-research.comen_GB
dc.subjectrheumatoid arthritisen_GB
dc.titleMonocytes/macrophages express CCR9 in rheumatoid arthritis and CCL25 stimulates their differentiationen
dc.typeArticleen
dc.contributor.departmentKeele University/University of Birmingham ; Keele University ; University of Chester ; University of Birmingham ; University of Chester ; University of Birmingham ; University of Birmingham ; University of Birmingham ; Keele University/University of Bristolen_GB
dc.identifier.journalArthritis Research & Therapyen_GB
dc.language.rfc3066enen
dc.rights.holderCaroline Schmutz et al.; licensee BioMed Central Ltd.en
dc.description.statusPeer Revieweden
dc.date.updated2013-03-15T10:30:18Zen
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