Hdl Handle:
http://hdl.handle.net/10034/263224
Title:
Measuring the secretion of heat shock proteins from cells
Authors:
Ireland, H. Elyse; Leoni, Francesca; Altaie, Ala; Birch, Catherine S.; Coleman, Robert C.; Hunter-Lavin, Claire; Williams, John H. H.
Abstract:
This article outlines procedures, using Hsp70 as the example, to: ensure the status of cells (viable, apoptotic or necrotic); identify the heat shock protein secreted; and quantify the secreted protein. Hsp70 has previously been quantified by ELISA, but newer methods are now being adopted, such as BIAcore and bead-based assays for use by FACS. These methods have the advantages of being more sensitive and requiring less sample than ELISA. The BIAcore has the potential to analyse Hsp70 ligands and provide affinity constants.
Affiliation:
University of Chester
Citation:
Methods, 43(3), 2007, pp. 176-183
Publisher:
Elsevier
Journal:
Methods
Publication Date:
2007
URI:
http://hdl.handle.net/10034/263224
DOI:
10.1016/j.ymeth.2007.06.011
Additional Links:
http://www.journals.elsevier.com/methods
Type:
Article
Language:
en
Description:
This article is not available through ChesterRep.
ISSN:
1046-2023
Appears in Collections:
Biological Sciences

Full metadata record

DC FieldValue Language
dc.contributor.authorIreland, H. Elyseen_GB
dc.contributor.authorLeoni, Francescaen_GB
dc.contributor.authorAltaie, Alaen_GB
dc.contributor.authorBirch, Catherine S.en_GB
dc.contributor.authorColeman, Robert C.en_GB
dc.contributor.authorHunter-Lavin, Claireen_GB
dc.contributor.authorWilliams, John H. H.en_GB
dc.date.accessioned2012-12-19T14:05:11Zen
dc.date.available2012-12-19T14:05:11Zen
dc.date.issued2007en
dc.identifier.citationMethods, 43(3), 2007, pp. 176-183en_GB
dc.identifier.issn1046-2023en
dc.identifier.doi10.1016/j.ymeth.2007.06.011en
dc.identifier.urihttp://hdl.handle.net/10034/263224en
dc.descriptionThis article is not available through ChesterRep.en_GB
dc.description.abstractThis article outlines procedures, using Hsp70 as the example, to: ensure the status of cells (viable, apoptotic or necrotic); identify the heat shock protein secreted; and quantify the secreted protein. Hsp70 has previously been quantified by ELISA, but newer methods are now being adopted, such as BIAcore and bead-based assays for use by FACS. These methods have the advantages of being more sensitive and requiring less sample than ELISA. The BIAcore has the potential to analyse Hsp70 ligands and provide affinity constants.en_GB
dc.language.isoenen
dc.publisherElsevieren_GB
dc.relation.urlhttp://www.journals.elsevier.com/methodsen_GB
dc.rightsArchived with thanks to Methodsen_GB
dc.subjectheat shock proteinsen_GB
dc.subjectHsp70 secretionen_GB
dc.subjectHsp60 secretionen_GB
dc.subjectnecrosisen_GB
dc.subjectapoptosisen_GB
dc.subjectHsp70 ELISAen_GB
dc.titleMeasuring the secretion of heat shock proteins from cellsen
dc.typeArticleen
dc.contributor.departmentUniversity of Chesteren_GB
dc.identifier.journalMethodsen_GB
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