Platelet-derived growth factor stimulates osteoprotegerin production in osteoblastic cells

Hdl Handle:
http://hdl.handle.net/10034/208689
Title:
Platelet-derived growth factor stimulates osteoprotegerin production in osteoblastic cells
Authors:
McCarthy, Helen S.; Williams, John H. H.; Davie, Michael W. J.; Marshall, Michael J.
Abstract:
This article discusses how osteoprotegerin (OPG) production by osteoblastic cells was stimulated by platelet-derived growth factor (PDGF) in two human osteosarcoma cell lines (MG63, Saos-2), a mouse pre-osteoblastic cell line (MC3T3-E1) and human bone marrow stromal cells (hMSC) by 152%, 197%, 113% and 45% respectively over 24 h. OPG was measured in the cell culture medium by immunoassay. PDGF isoforms AA, BB and AB show similar stimulation of OPG production. Message for OPG was also increased similarly to the increased secretion into the culture medium. Using specific inhibitors of cell signalling the authors demonstrate that PDGF acts through the PDGF receptor, PKC, PI3K, ERK and P38 and not via NF-kB or JNK. The importance of PDGF in fracture healing suggests a role for OPG production in countering bone resorption during the early phase of this process.
Affiliation:
Charles Salt Centre, Robert Jones and Agnes Hunt Orthopaedic Hospital NHS Trust in Oswestry / University of Chester ; University of Chester ; Charles Salt Centre, Robert Jones and Agnes Hunt Orthopaedic Hospital NHS Trust in Oswestry ; Charles Salt Centre, Robert Jones and Agnes Hunt Orthopaedic Hospital NHS Trust in Oswestry
Citation:
Journal of Cellular Physiology, 218(2), 2009, pp. 350-354
Publisher:
Wiley
Journal:
Journal of Cellular Physiology
Publication Date:
Feb-2009
URI:
http://hdl.handle.net/10034/208689
DOI:
10.1002/jcp.21600
Additional Links:
http://doi.wiley.com/10.1002/jcp.21600
Type:
Article
Language:
en
Description:
This article is not available through ChesterRep.
ISSN:
0021-9541; 1097-4652
Sponsors:
The Bone Disease Foundation
Appears in Collections:
Biological Sciences

Full metadata record

DC FieldValue Language
dc.contributor.authorMcCarthy, Helen S.en
dc.contributor.authorWilliams, John H. H.en
dc.contributor.authorDavie, Michael W. J.en
dc.contributor.authorMarshall, Michael J.en
dc.date.accessioned2012-02-03T10:13:32Zen
dc.date.available2012-02-03T10:13:32Zen
dc.date.issued2009-02en
dc.identifier.citationJournal of Cellular Physiology, 218(2), 2009, pp. 350-354en
dc.identifier.issn0021-9541en
dc.identifier.issn1097-4652en
dc.identifier.doi10.1002/jcp.21600en
dc.identifier.urihttp://hdl.handle.net/10034/208689en
dc.descriptionThis article is not available through ChesterRep.en
dc.description.abstractThis article discusses how osteoprotegerin (OPG) production by osteoblastic cells was stimulated by platelet-derived growth factor (PDGF) in two human osteosarcoma cell lines (MG63, Saos-2), a mouse pre-osteoblastic cell line (MC3T3-E1) and human bone marrow stromal cells (hMSC) by 152%, 197%, 113% and 45% respectively over 24 h. OPG was measured in the cell culture medium by immunoassay. PDGF isoforms AA, BB and AB show similar stimulation of OPG production. Message for OPG was also increased similarly to the increased secretion into the culture medium. Using specific inhibitors of cell signalling the authors demonstrate that PDGF acts through the PDGF receptor, PKC, PI3K, ERK and P38 and not via NF-kB or JNK. The importance of PDGF in fracture healing suggests a role for OPG production in countering bone resorption during the early phase of this process.en
dc.description.sponsorshipThe Bone Disease Foundationen
dc.language.isoenen
dc.publisherWileyen
dc.relation.urlhttp://doi.wiley.com/10.1002/jcp.21600en
dc.subjectosteoprotegerin (OPG)en
dc.subjectplatelet-derived growth factor (PDGF)en
dc.titlePlatelet-derived growth factor stimulates osteoprotegerin production in osteoblastic cellsen
dc.typeArticleen
dc.contributor.departmentCharles Salt Centre, Robert Jones and Agnes Hunt Orthopaedic Hospital NHS Trust in Oswestry / University of Chester ; University of Chester ; Charles Salt Centre, Robert Jones and Agnes Hunt Orthopaedic Hospital NHS Trust in Oswestry ; Charles Salt Centre, Robert Jones and Agnes Hunt Orthopaedic Hospital NHS Trust in Oswestryen
dc.identifier.journalJournal of Cellular Physiologyen
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