Welcome to ChesterRep - the University of Chester's Online Research Repository
ChesterRep is the University of Chester's institutional repository and an online platform designed to collate, store, and aid discoverability of the University’s research.
All University of Chester staff are expected to use the Current Research Information System, Symplectic Elements, to submit material to ChesterRep. Guidance on how to deposit and manage publications using Elements can be found here. You can also discover more about our editorial and open access policies here. Please note that you must be a member of the University to view these pages.
If you are a student at the University of Chester and want to submit work to ChesterRep, please contact researchsupport.lis@chester.ac.uk.
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FTO rs17817449 variant increases the risk of severe obesity in a Brazilian cohort: A case-control studyPURPOSE: Obesity is a complex disease caused by a combination of genetic, environmental, and epigenetic factors, and is associated with an increased risk of chronic diseases. The leptin-melanocortin pathway integrates peripheral signals about the body's energy stores with a central neuronal circuit in the hypothalamus. This pathway has been extensively studied over the years, as genetic variations in genes related to it may play a crucial role in determining an individual's susceptibility to obesity. Therefore, we analyzed the association between obesity and specific polymorphisms in leptin-melanocortin-related genes such as LEPR rs1137101, POMC rs1042571, LEP rs7799039, BDNF rs6265, FTO rs17817449, CART rs121909065, and NPY rs16147/rs5574. PATIENTS AND METHODS: The study enrolled 501 participants from Rio de Janeiro, Brazil, with obesity class II or greater (BMI ≥ 35 kg/m2) and normal weight controls (18.5≤ BMI ≤24.9 kg/m2). We collected demographic, body composition, biochemical, and genotyping data by real-time PCR, and performed logistic and linear regression analyses to investigate the association of polymorphisms with severe obesity status and obesity-related quantitative parameters. RESULTS: Individuals with severe obesity had significantly higher anthropometric measures, blood pressure, and biochemical levels. The FTO rs17817449 TT genotype was associated with a significantly higher risk of developing severe obesity, and distinct cytokine expression was observed across the FTO rs17817449 genotypes. The BDNF rs6265 dominant-model and NPY rs16147 CC genotypes were associated with triglyceride levels and childhood obesity, respectively. Finally, individuals with obesity were more likely to carry a greater number of risk alleles than those without obesity. CONCLUSION: Our study observed an important association between FTO rs17817449 polymorphism with obesity and obesity-related traits. Additionally, BDNF rs6265 dominant-model was associated with triglyceride serum levels, and NPY rs16147 may have a role in obesity onset.
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Assessing risk factors for drug storage practices in veterinary surgeries: A questionnaire study of UK veterinary professionals.BackgroundSafe drug storage practices are essential in veterinary practice to maintain optimal standards of animal care. Practical challenges in clinic may impact their uptake, which could affect drug efficacy and the success of treatment. The UK is presumed to provide high standards for animal care and welfare in the veterinary profession and may provide an interesting case study to assess veterinary drug storage practices.MethodsAn online survey with 184 practice participants assessed UK veterinary professionals' responses on drug storage practices. This included socio-demographic information and questions or statements that examined storage practices compliant with the requirements established by the Royal College of Veterinary Surgeons (RCVS), the regulatory body for veterinary practice in the UK.ResultsOverall, practices followed RCVS-recommended measures, though there was clear selectivity for stock temperature checks (72.2%), over other measures, particularly, replacing stock (54.4%) and returning medications to refrigerated storage (52.5%). Clinical experience and practice type impacted most on drug storage practices, with more experienced clinicians and small animal practices showing greater uptake of best measures.ConclusionsWe suggest that practices should adopt all of the RCVS measures in relation to veterinary drug storage practices. Socio-demographic factors should be considered because they can act as risk factors influencing best practice in clinics. Our findings may have wider implications for the veterinary profession in general, given similar demographic trends in veterinary practice in many European countries.
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Anti-inflammatory effects of Phyllanthus amarus extracts against Benzene-induced Leukemia in ratsBACKGROUND: The present study examined the protective effects of extracts from Phyllanthus amarus on benzene-induced leukemia in Wistar rats. Benzene is a carcinogen linked to increased leukemia risk. OBJECTIVES: The study aimed to assess the impact of P. amarus extracts, prepared via different drying methods, on immunological, biochemical, and histopathological parameters. METHODS: Aqueous, methanolic, and ethanolic extracts were prepared from P. amarus using room drying, oven drying, and sun drying. The rats were treated with benzene and the extracts. For the immunological parameters, C-reactive protein (CRP), interleukin-8 (IL-8), transforming growth factor-beta (TGF-β), tumor necrosis factor-alpha (TNF-α), and IL-10 were measured using the enzyme-linked immunosorbent assay. For biochemical parameters, microalbumin, urea, creatinine, alanine aminotransferase (ALT), alkaline phosphatase (ALP), and aspartate aminotransferase (AST) were assessed using spectrophotometry. At the same time, for histopathological examination, liver and bone marrow tissues were stained using hematoxylin and eosin and analyzed for morphological changes. RESULTS: Research findings showed no significant difference in CRP among the groups (P=0.197), indicating no significant inflammation or tissue damage. TGF-β levels were significantly lower in treatment groups compared to the positive control group (P=0.015), suggesting anti-inflammatory or immunosuppressive effects. No significant differences were found in IL-8, TNF-α, and IL-10 levels. The aqueous extract prepared by room drying significantly decreased microalbumin levels (P=0.016), representing potential protective effects on kidney function. The methanolic extract prepared by sun drying significantly reduced creatinine (P=0.032) and ALT (P=0.048) levels, implying beneficial effects on liver function. Histopathological examinations revealed that the extracts modulated bone marrow and liver morphologies, reducing inflammation while improving cellularity and morphology. CONCLUSION: P. amarus extracts demonstrated potential anti-inflammatory effects in benzene-induced leukemia by significantly reducing TGF-β levels without inducing inflammation, as evidenced by stable CRP, IL-8, and TNF-α levels. These findings suggest that the extracts may help mitigate inflammation associated with benzene exposure, highlighting their potential as adjunctive therapies in leukemia treatment. More studies are needed to understand these protective processes completely and investigate their clinical uses.
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Creative Health a joke or valuable learning experience; A mixed methods studyBACKGROUND: Creative Health has been recognised to be beneficial for wellbeing and population health. Recommendations have been made that health care students and professionals should receive education and practical experience of the arts. This paper reports on a pilot creative health placement for undergraduate nurses at a UK University. OBJECTIVE: To evaluate a Creative Health placement, investigating the placements impact on student's knowledge and understanding of arts-based activities, including social prescribing. To reflect on the overall value of Creative Health placements for nursing students and to make recommendations for future Creative Health placements. DESIGN: A mixed methods approach was adopted using the principles of ethnography to evaluate the experiences of the students, artists, service users and practice assessors/supervisors. SETTING: Data was collected from one University Centre that facilitated the Creative Health placement. PARTICIPANTS: 60 Bachelor of Nursing Students, 4 practice assessors/supervisors and 6 Creative Health artists and 89 service users participated. METHODS: Ethnographic data, including artefacts, photographs, diaries, qualitative reflections were collected alongside a quantitative evaluation survey that students completed. Service user feedback forms were used to collect user experiences and two focus groups for the artist and practice assessor were employed using thematic analysis. RESULTS: The placement provided the students insights and understanding about creative health and social prescribing, however there were challenges regarding conceptualising creative health as part of their nursing practice. Students talked about the impact the placement had on their own wellbeing. 81.8 % agreed working with the artists provided insight into Creative Health practice and 86.4 % indicated the placement enabled them to understand the impact of Creative Health on wellbeing. Creative Health artists and the practice assessors/supervisors wanted greater collaboration to support learning and assessment. Service users provided positive feedback about their experiences. CONCLUSIONS: Overall, the placement was a success and there were valuable lessons learnt for future placement plans. We concluded that creative health placements should be an essential part of the nursing programme.
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LinkedIn as a research participant recruitment tool: reflections from the football industryPURPOSE: The article explores the process of using LinkedIn to recruit hard-to-reach groups, reflecting on our experience of the football industry. We propose LinkedIn as a viable option to mitigate recruitment challenges, particularly in employment-focussed research. DESIGN/METHODOLOGY/APPROACH: We examine how LinkedIn was used to recruit 43 football backroom staff for a sociological research study. It outlines the process of using LinkedIn to contact participants, the ethical considerations made throughout the research process and reflects on why LinkedIn was effective for a primarily employment-focussed study. FINDINGS: We discuss the importance of insider knowledge for identifying LinkedIn as a potentially fruitful recruitment tool and how the functionality of the platform can be beneficial for a targeted recruitment method. We also explore the ethical and practical considerations of using social media for recruitment. ORIGINALITY/VALUE: Previous research discusses social media as if they are indistinguishable and interchangeable with one another and we argue that this neglects the importance of a platform’s functionality. We discuss how the decision to use a particular social media platform to recruit should be grounded in the researcher’s familiarity with the site, the functionality the platform offers and the sample recruited. This article explicitly explores the considerations taken when using LinkedIn to help overcome recruitment challenges.