Measurement of bovine IgG by indirect competitive ELISA as a means of detecting milk adulteration

Hdl Handle:
http://hdl.handle.net/10034/70436
Title:
Measurement of bovine IgG by indirect competitive ELISA as a means of detecting milk adulteration
Authors:
Hurley, Ian P.; Coleman, Robert C.; Ireland, H. Elyse; Williams, John H. H.
Abstract:
The aim of this work was to develop an assay capable of detecting adulteration of high premium milk with milk from cheaper sources. An indirect, competitive ELISA was developed for the rapid detection of cows’ milk in the milk of goat, sheep, and buffalo. The assay uses a monoclonal antibody produced against bovine IgG. This antibody recognizes a species-specific epitope on the heavy chain of both bovine IgG1 and IgG2. A peroxidase-conjugated anti-mouse IgG antibody was used to detect bound monoclonal antibody and subsequent enzymatic conversion of substrate resulted in clear differences in absorbance when assaying different mixtures of milks adulterated with cows’ milk. Once optimized, the ELISA was found to be highly specific. Detection limits of the assay are 1.0 µg/mL of bovine IgG, or 0.1% (vol/vol) adulteration with cows’ milk. The assay was highly reproducible (CV < 10%) and performed equally well when used to detect bovine IgG in mixtures with the 3 types of milk tested. The ELISA performance makes it suitable for development as a kit, for use in the field as a high throughput screening ELISA.
Affiliation:
University College Chester
Citation:
Journal of Diary Science, 2004, 87(3), pp. 543-549.
Publisher:
American Dairy Science Association
Journal:
Journal of Diary Science
Publication Date:
Mar-2004
URI:
http://hdl.handle.net/10034/70436
DOI:
10.3168/jds.S0022-0302(04)73195-1
Additional Links:
http://jds.fass.org
Type:
Article
Language:
en
Description:
This article is not available through ChesterRep. It is available at http://download.journals.elsevierhealth.com/pdfs/journals/0022-0302/PIIS0022030204731951.pdf
Sponsors:
This article was submitted to the RAE2008 for the University of Chester - Allied Health Professions and Studies.
Appears in Collections:
Biological Sciences

Full metadata record

DC FieldValue Language
dc.contributor.authorHurley, Ian P.en
dc.contributor.authorColeman, Robert C.en
dc.contributor.authorIreland, H. Elyseen
dc.contributor.authorWilliams, John H. H.en
dc.date.accessioned2009-06-15T11:08:37Zen
dc.date.available2009-06-15T11:08:37Zen
dc.date.issued2004-03en
dc.identifier.citationJournal of Diary Science, 2004, 87(3), pp. 543-549.en
dc.identifier.doi10.3168/jds.S0022-0302(04)73195-1en
dc.identifier.urihttp://hdl.handle.net/10034/70436en
dc.descriptionThis article is not available through ChesterRep. It is available at http://download.journals.elsevierhealth.com/pdfs/journals/0022-0302/PIIS0022030204731951.pdfen
dc.description.abstractThe aim of this work was to develop an assay capable of detecting adulteration of high premium milk with milk from cheaper sources. An indirect, competitive ELISA was developed for the rapid detection of cows’ milk in the milk of goat, sheep, and buffalo. The assay uses a monoclonal antibody produced against bovine IgG. This antibody recognizes a species-specific epitope on the heavy chain of both bovine IgG1 and IgG2. A peroxidase-conjugated anti-mouse IgG antibody was used to detect bound monoclonal antibody and subsequent enzymatic conversion of substrate resulted in clear differences in absorbance when assaying different mixtures of milks adulterated with cows’ milk. Once optimized, the ELISA was found to be highly specific. Detection limits of the assay are 1.0 µg/mL of bovine IgG, or 0.1% (vol/vol) adulteration with cows’ milk. The assay was highly reproducible (CV < 10%) and performed equally well when used to detect bovine IgG in mixtures with the 3 types of milk tested. The ELISA performance makes it suitable for development as a kit, for use in the field as a high throughput screening ELISA.en
dc.description.sponsorshipThis article was submitted to the RAE2008 for the University of Chester - Allied Health Professions and Studies.en
dc.language.isoenen
dc.publisherAmerican Dairy Science Associationen
dc.relation.urlhttp://jds.fass.orgen
dc.subjectmilk adulterationen
dc.subjectELISAen
dc.subjectIgGen
dc.titleMeasurement of bovine IgG by indirect competitive ELISA as a means of detecting milk adulterationen
dc.typeArticleen
dc.contributor.departmentUniversity College Chesteren
dc.identifier.journalJournal of Diary Scienceen
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