Hdl Handle:
http://hdl.handle.net/10034/69814
Title:
Hsp70 release from peripheral blood mononuclear cells
Authors:
Hunter-Lavin, Claire; Davies, Emma L.; Bacelar, Maria M. F. V. G.; Marshall, Michael J.; Andrew, Sarah M.; Williams, John H. H.
Abstract:
There are an increasing number of studies reporting the presence of Hsps in human serum. We have investigated the release of Hsp70 into blood and culture medium from peripheral blood mononuclear cells (PBMCs), and whether this release is due to cell damage or active secretion from the cells. Intact Hsp70 was released from cells within whole blood and from purified PBMCs under normal culture conditions. Hsp70 release was rapid (0.1 ng/106 cells/h) over the first 2 h of culture and continued at a reduced rate up to 24 h (<0.025 ng/106 cells/h). Using viable cell counts and lactate dehydrogenase release we were able to confirm that the release of Hsp70 was not due to cellular damage. Hsp70 release was inhibited by monensin, methyl-β-cyclodextrin, and methylamine, but not by brefeldin A. These data suggest that Hsp70 is released from cells via a non-classical pathway, possibly involving lysosomal lipid rafts.
Affiliation:
University of Chester ; University of Chester ; University of Chester ; The Robert Jones and Agnes Hunt Orthopaedic Hospital, Oswestry ; University College Chester ; University College Chester
Citation:
Biochemical and Biophysical Research Communications, 324(2), 2004, pp. 511-517
Publisher:
Elsevier
Journal:
Biochemical and Biophysical Research Communications
Publication Date:
12-Nov-2004
URI:
http://hdl.handle.net/10034/69814
DOI:
10.1016/j.bbrc.2004.09.075
Additional Links:
http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description#description
Type:
Article
Language:
en
Description:
This article is not available through ChesterRep.
ISSN:
0006-291X; 1090-2104
Sponsors:
This article was submitted to the RAE2008 for the University of Chester - Allied Health Professions and Studies.
Appears in Collections:
Biological Sciences

Full metadata record

DC FieldValue Language
dc.contributor.authorHunter-Lavin, Claireen
dc.contributor.authorDavies, Emma L.en
dc.contributor.authorBacelar, Maria M. F. V. G.en
dc.contributor.authorMarshall, Michael J.en
dc.contributor.authorAndrew, Sarah M.en
dc.contributor.authorWilliams, John H. H.en
dc.date.accessioned2009-06-05T16:20:11Zen
dc.date.available2009-06-05T16:20:11Zen
dc.date.issued2004-11-12en
dc.identifier.citationBiochemical and Biophysical Research Communications, 324(2), 2004, pp. 511-517en
dc.identifier.issn0006-291Xen
dc.identifier.issn1090-2104en
dc.identifier.doi10.1016/j.bbrc.2004.09.075en
dc.identifier.urihttp://hdl.handle.net/10034/69814en
dc.descriptionThis article is not available through ChesterRep.en
dc.description.abstractThere are an increasing number of studies reporting the presence of Hsps in human serum. We have investigated the release of Hsp70 into blood and culture medium from peripheral blood mononuclear cells (PBMCs), and whether this release is due to cell damage or active secretion from the cells. Intact Hsp70 was released from cells within whole blood and from purified PBMCs under normal culture conditions. Hsp70 release was rapid (0.1 ng/106 cells/h) over the first 2 h of culture and continued at a reduced rate up to 24 h (<0.025 ng/106 cells/h). Using viable cell counts and lactate dehydrogenase release we were able to confirm that the release of Hsp70 was not due to cellular damage. Hsp70 release was inhibited by monensin, methyl-β-cyclodextrin, and methylamine, but not by brefeldin A. These data suggest that Hsp70 is released from cells via a non-classical pathway, possibly involving lysosomal lipid rafts.en
dc.description.sponsorshipThis article was submitted to the RAE2008 for the University of Chester - Allied Health Professions and Studies.en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttp://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description#descriptionen
dc.subjectHsp70en
dc.subjectperipheral blood mononuclear cellsen
dc.subjectlymphocytesen
dc.subjectnon-classical protein secretionen
dc.titleHsp70 release from peripheral blood mononuclear cellsen
dc.typeArticleen
dc.contributor.departmentUniversity of Chester ; University of Chester ; University of Chester ; The Robert Jones and Agnes Hunt Orthopaedic Hospital, Oswestry ; University College Chester ; University College Chesteren
dc.identifier.journalBiochemical and Biophysical Research Communicationsen
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